Regulation of hepatitis C virus translation initiation by iron: Role of eIF3 and La protein

• Published in: Virus research Vol. 167; no. 2; pp. 302 - 309
• Main Authors: Wang, Qiang, Liu, Yiping, An, Daizhi, Diao, Hongyan, Xu, Wei, He, Xiang, Sun, Rubao, Wei, Li, Li, Lanjuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2012
• Subjects: Blotting, Western; Cell Line; Eukaryotic initiation factor; Eukaryotic Initiation Factor-3 - metabolism; Gene Expression Profiling; Genes, Reporter; Hepacivirus - physiology; Hepatitis C virus; Hepatocytes - virology; Host-Pathogen Interactions; Humans; Internal ribosome-entry site; Iron; Iron - metabolism; Phosphoproteins - metabolism; Protein Biosynthesis; Real-Time Polymerase Chain Reaction; PCBP; HCC; Iron; RLuc; IFN; Internal ribosome-entry site; 5′-UTR; FLuc; IRES; PTBP; DFX; HCV; Hepatitis C virus; Eukaryotic initiation factor; eIFs; RNPL

► Iron is positively associated with the HCV IRES-dependent translation. ► Iron enhances the expression of eIF3A and La proteins. ► Inhibition of eIF3A and La substantially repress iron-dependent HCV translation. Eukaryotic initiation factors (eIFs) are required for encoding polyprotein of hepatitis...