The degradation of allyl isothiocyanate and its impact on methane production from anaerobic co-digestion of kitchen waste and waste activated sludge

[Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation mic...

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Published inBioresource technology Vol. 347; p. 126366
Main Authors Qin, Yu, Yang, Jingnan, Wu, Yanxin, Wang, Dongbo, Liu, Xuran, Du, Mingting, He, Dandan, Yi, Neng
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.03.2022
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Abstract [Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation microbes such as Bacillus. Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.
AbstractList Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.
Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.
Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.
[Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation microbes such as Bacillus. Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.
ArticleNumber 126366
Author Yi, Neng
Yang, Jingnan
Liu, Xuran
He, Dandan
Wu, Yanxin
Wang, Dongbo
Du, Mingting
Qin, Yu
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Keywords Kitchen waste
Anaerobic co-digestion
Waste activated sludge
Allyl isothiocyanate
Methane production
Language English
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Snippet [Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion...
Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the...
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StartPage 126366
SubjectTerms activated sludge
Allyl isothiocyanate
anaerobes
Anaerobic co-digestion
anaerobic digestion
Anaerobiosis
apoptosis
Bioreactors
Brassicaceae
cell membranes
Digestion
hydrolysis
Isothiocyanates
Kitchen waste
Methane
Methane production
Sewage
Waste activated sludge
Waste Disposal, Fluid
Title The degradation of allyl isothiocyanate and its impact on methane production from anaerobic co-digestion of kitchen waste and waste activated sludge
URI https://dx.doi.org/10.1016/j.biortech.2021.126366
https://www.ncbi.nlm.nih.gov/pubmed/34838636
https://www.proquest.com/docview/2604470107
https://www.proquest.com/docview/2636441573
Volume 347
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