The degradation of allyl isothiocyanate and its impact on methane production from anaerobic co-digestion of kitchen waste and waste activated sludge
[Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation mic...
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Published in | Bioresource technology Vol. 347; p. 126366 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.03.2022
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Abstract | [Display omitted]
•AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation microbes such as Bacillus.
Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis. |
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AbstractList | Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis.Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis. Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis. Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis. [Display omitted] •AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion of KW and WAS.•AITC inhibited acetotrophic methanogenesis by reducing the activity of AK enzyme.•AITC enriched complex organic degradation microbes such as Bacillus. Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the fate and impact of allyl isothiocyanate (AITC), a main active component in cruciferous vegetables, in the anaerobic co-digestion has never been documented. This study therefore aims to provide such supports. Experimental results exhibited that AITC was degraded completely by microorganisms and served as a substrate to produce methane. As AITC increased from 0 to 60 mg/L, the maximum methane production decreased from 285.1 to 35.8 mL/g VS, and the optimum digestion time was also prolonged. The mechanism study demonstrated that AITC induced cell apoptosis by modifying the physicochemical properties of cell membrane, which resulted in inhibitions to the procedure of anaerobic co-digestion. The high-throughput sequencing showed that AITC enriched the microorganism for degradation of complex organic compounds such as Bacillus, but lessened anaerobes involved in hydrolysis, acidogenesis, and methanogenesis. |
ArticleNumber | 126366 |
Author | Yi, Neng Yang, Jingnan Liu, Xuran He, Dandan Wu, Yanxin Wang, Dongbo Du, Mingting Qin, Yu |
Author_xml | – sequence: 1 givenname: Yu surname: Qin fullname: Qin, Yu organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 2 givenname: Jingnan surname: Yang fullname: Yang, Jingnan organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 3 givenname: Yanxin surname: Wu fullname: Wu, Yanxin organization: College of Environmental Science and Engineering, Xiangtan University, Xiangtan 411105, China – sequence: 4 givenname: Dongbo surname: Wang fullname: Wang, Dongbo email: w.dongbo@yahoo.com organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 5 givenname: Xuran surname: Liu fullname: Liu, Xuran organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 6 givenname: Mingting surname: Du fullname: Du, Mingting organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 7 givenname: Dandan surname: He fullname: He, Dandan organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China – sequence: 8 givenname: Neng surname: Yi fullname: Yi, Neng organization: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34838636$$D View this record in MEDLINE/PubMed |
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Keywords | Kitchen waste Anaerobic co-digestion Waste activated sludge Allyl isothiocyanate Methane production |
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•AITC was degraded by –NCS and CC–C– isomerization in anaerobic co-digestion.•AITC inhibited methane production from anaerobic co-digestion... Producing methane from anaerobic co-digestion of kitchen waste and waste activated sludge has been widely implemented in real-world situations. However, the... |
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SubjectTerms | activated sludge Allyl isothiocyanate anaerobes Anaerobic co-digestion anaerobic digestion Anaerobiosis apoptosis Bioreactors Brassicaceae cell membranes Digestion hydrolysis Isothiocyanates Kitchen waste Methane Methane production Sewage Waste activated sludge Waste Disposal, Fluid |
Title | The degradation of allyl isothiocyanate and its impact on methane production from anaerobic co-digestion of kitchen waste and waste activated sludge |
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