Subphysiological Compressive Loading Reduces Apoptosis Following Acute Impact Injury in a Porcine Cartilage Model
Background: Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine a...
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| Published in | Sports health Vol. 6; no. 1; pp. 81 - 88 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Los Angeles, CA
SAGE Publications
01.01.2014
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Background:
Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs.
Hypothesis:
Compressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury.
Study Design:
In vitro controlled laboratory study.
Level of Evidence:
Level 5.
Methods:
Each experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction.
Results:
Cell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions.
Conclusion:
In this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability.
Clinical Relevance:
Therapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury. |
|---|---|
| AbstractList | Background:
Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs.
Hypothesis:
Compressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury.
Study Design:
In vitro controlled laboratory study.
Level of Evidence:
Level 5.
Methods:
Each experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction.
Results:
Cell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions.
Conclusion:
In this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability.
Clinical Relevance:
Therapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury. BACKGROUNDAcute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs.HYPOTHESISCompressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury.STUDY DESIGNIn vitro controlled laboratory study.LEVEL OF EVIDENCELevel 5.METHODSEach experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction.RESULTSCell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions.CONCLUSIONIn this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability.CLINICAL RELEVANCETherapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury. Background:Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs.Hypothesis:Compressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury.Study Design:In vitro controlled laboratory study.Level of Evidence:Level 5.Methods:Each experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction.Results:Cell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions.Conclusion:In this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability.Clinical Relevance:Therapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury. Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this study was to investigate the effect of posttraumatic cyclic compressive loading on chondrocyte viability and apoptosis in porcine articular cartilage plugs. Compressive loading of acutely injured cartilage can maintain chondrocyte viability by reducing apoptosis after a traumatic impact injury. In vitro controlled laboratory study. Level 5. Each experiment compared 4 test groups: control, impact, impact with compressive loading (either 0.5 or 0.8 MPa), and no impact but compressive loading (n = 15 per group). Flat, full-thickness articular cartilage plugs were harvested from the trochlear region of porcine knees. A drop tower was utilized to introduce an impact injury. The articular plugs were subjected to two 30-minute cycles of either 0.5 or 0.8 MPa of dynamic loading. Cell viability, apoptosis, and gene expression of samples were evaluated 24 hours postimpaction. Cell viability staining showed that 0.5 MPa of dynamic compressive loading increased cell viability compared with the impact group. Apoptotic analysis revealed a decrease in apoptotic expression in the group with 0.5 MPa of dynamic compressive loading compared with the impact group. Significantly higher caspase 3 and lower collagen II expressions were observed in impacted samples without compressive loading, compared with those with. Compressive loading of nonimpacted samples significantly increased collagen II and decreased caspase 3 expressions. In this porcine in vitro model, dynamic compressive loading at subphysiological levels immediately following impact injury decreases apoptotic expression, thereby maintaining chondrocyte viability. Therapeutic exercises could be designed to deliver subphysiological loading to the injured cartilage, thereby minimizing injury. |
| Author | Abadin, Andre Wilensky, David Kaplan, Lee Huang, C.-Y. Charles Vernon, Lauren |
| AuthorAffiliation | Department of Biomedical Engineering, University of Miami, Coral Gables, Florida Division of Sports Medicine, UHealth Sports Performance and Wellness Institute, University of Miami Hospital, Miami, Florida |
| AuthorAffiliation_xml | – name: Department of Biomedical Engineering, University of Miami, Coral Gables, Florida – name: Division of Sports Medicine, UHealth Sports Performance and Wellness Institute, University of Miami Hospital, Miami, Florida |
| Author_xml | – sequence: 1 givenname: Lauren surname: Vernon fullname: Vernon, Lauren – sequence: 2 givenname: Andre surname: Abadin fullname: Abadin, Andre – sequence: 3 givenname: David surname: Wilensky fullname: Wilensky, David – sequence: 4 givenname: C.-Y. Charles surname: Huang fullname: Huang, C.-Y. Charles email: c.huang@miami.edu – sequence: 5 givenname: Lee surname: Kaplan fullname: Kaplan, Lee |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24427447$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_isci_2024_109983 crossref_primary_10_1016_j_jbiomech_2021_110736 crossref_primary_10_1016_j_joca_2015_05_010 crossref_primary_10_1177_0300985815588611 crossref_primary_10_3390_ijms22073726 crossref_primary_10_1002_jor_23086 crossref_primary_10_3390_bioengineering11020110 crossref_primary_10_1177_1947603515618483 crossref_primary_10_3389_fbioe_2022_886360 |
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Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The... Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective of this... Background: Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The... BACKGROUNDAcute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective... Background:Acute cartilage injuries induce cell death and are associated with an increased incidence of osteoarthritis development later in life. The objective... |
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| SubjectTerms | Orthopaedic Surgery |
| Title | Subphysiological Compressive Loading Reduces Apoptosis Following Acute Impact Injury in a Porcine Cartilage Model |
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