Borna disease virus phosphoprotein triggers the organization of viral inclusion bodies by liquid-liquid phase separation

• Published in: International journal of biological macromolecules Vol. 192; pp. 55 - 63
• Main Authors: Hirai, Yuya, Tomonaga, Keizo, Horie, Masayuki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2021
• Subjects: Animals; Biomolecular condensates; Biomolecular Condensates - metabolism; Biomolecular Condensates - pathology; Borna Disease - metabolism; Borna Disease - pathology; Borna Disease - virology; Borna disease virus; Borna disease virus - physiology; Cell Fractionation - methods; cell nucleus; Cells, Cultured; chronic diseases; Fluorescent Antibody Technique; Inclusion Bodies, Viral - metabolism; Inclusion Bodies, Viral - pathology; Intrinsically disordered regions; Liquid droplets; Liquid-Liquid Extraction; Liquid-liquid phase separation; liquids; Membraneless organelles; Microscopy, Confocal; Mononegavirus; Non-segmented negative-strand RNA viruses; Nucleoprotein; nucleoproteins; Nucleus; Phosphoprotein; phosphoproteins; Phosphoproteins - metabolism; RNA; separation; Viral Proteins - metabolism; Nucleus; Liquid droplets; Borna disease virus; Non-segmented negative-strand RNA viruses; Intrinsically disordered regions; Membraneless organelles; Nucleoprotein; Mononegavirus; Phosphoprotein; Liquid-liquid phase separation; Biomolecular condensates

Inclusion bodies (IBs) are characteristic biomolecular condensates organized by the non-segmented negative-strand RNA viruses belonging to the order Mononegavirales. Although recent studies have revealed the characteristics of IBs formed by cytoplasmic mononegaviruses, that of Borna disease virus 1...