Epidermal Growth Factor-Induced Selective Phosphorylation of Cultured Rat Hepatocyte 55-kD Cytokeratin before Filament Reorganization and DNA Synthesis

• Published in: The Journal of cell biology Vol. 109; no. 4; pp. 1665 - 1676
• Main Authors: Baribault, Helene, Blouin, Richard, Bourgon, Lise, Marceau, Normand
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.10.1989; The Rockefeller University Press
• Subjects: Actin Cytoskeleton - drug effects; Actin Cytoskeleton - ultrastructure; Animals; Biological and medical sciences; Cell structures and functions; Cells; Cells, Cultured; Cultured cells; Cytoskeleton - ultrastructure; Cytoskeleton, cytoplasm. Intracellular movements; Dexamethasone - pharmacology; DNA Replication - drug effects; Embryonic cells; Epidermal Growth Factor - pharmacology; Epithelial cells; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Hepatocytes; Insulin; Keratins; Keratins - biosynthesis; Keratins - metabolism; Kinetics; Liver - drug effects; Liver - metabolism; Liver - ultrastructure; Liver cells; Molecular and cellular biology; Molecular Weight; Phosphoproteins - biosynthesis; Phosphoproteins - isolation & purification; Phosphorylation; Rats; Rats, Inbred F344; Cell culture; Phosphorylation; Intermediary filament; Radiolabelling; Rat; Mitosis; Rodentia; Microfilament; DNA synthesis; Indirect immunofluorescence; Cell differentiation; Proteins; Keratin; Vertebrata; Mammalia; Epidermal growth factor; Hepatocyte; Aminoacid; Immunoblotting assay; Molecular rearrangement; Cytoskeleton; Microtubule; Fluorescence microscopy

We have reported previously that the addition of dexamethasone to cultured quiescent suckling rat hepatocytes in the presence of insulin, a culture condition which does not cause growth activation, induces a selective increase in the synthesis of the 49-kD/55-kD cytokeratin (CK49/CK55) pair over a 2...