Ligand-specific Conformation of Extracellular Loop-2 in the Angiotensin II Type 1 Receptor

• Published in: The Journal of biological chemistry Vol. 285; no. 21; pp. 16341 - 16350
• Main Authors: Unal, Hamiyet, Jagannathan, Rajaganapathi, Bhat, Manjunatha B., Karnik, Sadashiva S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.05.2010; American Society for Biochemistry and Molecular Biology
• Subjects: 7-Helix Receptor; Ang II; Angiotensin II Type 1 Receptor Blockers - chemistry; Angiotensin II Type 1 Receptor Blockers - metabolism; Animals; AT1R; Biotinylation; Chlorocebus aethiops; Cooperativity; COS Cells; Disulfides; Drug Action; ECL2; G-Protein-coupled Receptors (GPCR); G-Proteins; Ligand-induced Conformational Change; Ligands; Mutation, Missense; Peptide Mapping; Protein Binding; Protein Structure and Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Receptor, Angiotensin, Type 1 - agonists; Receptor, Angiotensin, Type 1 - chemistry; Receptor, Angiotensin, Type 1 - genetics; Receptor, Angiotensin, Type 1 - metabolism; Rhodopsin - chemistry; Rhodopsin - genetics; Rhodopsin - metabolism; Signal Transduction; Streptavidin - chemistry; Structural Homology, Protein; G-Proteins; ECL2; Drug Action; G-Protein-coupled Receptors (GPCR); Ligand-induced Conformational Change; 7-Helix Receptor; AT1R; Cooperativity; Ang II

The orientation of the second extracellular loop (ECL2) is divergent in G-protein coupled receptor (GPCR) structures determined. This discovery provoked the question, is the ECL2 conformation differentially regulated in the GPCRs that respond to diffusible ligands? We have determined the conformatio...