Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3

• Published in: Viruses Vol. 9; no. 3; p. 44
• Main Authors: Rausch, Jason, Miller, Jennifer, Le Grice, Stuart
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.03.2017; MDPI
• Subjects: Binding sites; DNA polymerase; Enzymes; Genomes; HIV; Human immunodeficiency virus; Insects; Laboratories; Leukemia; Medical research; Mutation; Nucleic acids; retroelement; Retroelements; retrotransposon; reverse transcriptase; Reverse Transcription; Review; ribonuclease H (RNase H); RNA-Directed DNA Polymerase - chemistry; RNA-Directed DNA Polymerase - genetics; RNA-Directed DNA Polymerase - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Terminal Repeat Sequences; Transfer RNA; Ty3; Viruses; retroelement; DNA polymerase; reverse transcriptase; retrotransposon; reverse transcription; Ty3; ribonuclease H (RNase H)

Converting the single-stranded retroviral RNA into integration-competent double-stranded DNA is achieved through a multi-step process mediated by the virus-coded reverse transcriptase (RT). With the exception that it is restricted to an intracellular life cycle, replication of the Saccharomyces cere...