Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions

• Published in: Cell stem cell Vol. 20; no. 6; pp. 858 - 873.e4
• Main Authors: Li, Li, Dong, Ji, Yan, Liying, Yong, Jun, Liu, Xixi, Hu, Yuqiong, Fan, Xiaoying, Wu, Xinglong, Guo, Hongshan, Wang, Xiaoye, Zhu, Xiaohui, Li, Rong, Yan, Jie, Wei, Yuan, Zhao, Yangyu, Wang, Wei, Ren, Yixin, Yuan, Peng, Yan, Zhiqiang, Hu, Boqiang, Guo, Fan, Wen, Lu, Tang, Fuchou, Qiao, Jie
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2017
• Subjects: Bone Morphogenetic Proteins - metabolism; Cell Division - physiology; Embryonic Germ Cells - cytology; Embryonic Germ Cells - metabolism; Female; Fetus - cytology; Fetus - metabolism; germ cell; germline; gonad; Gonads - cytology; Gonads - enzymology; granulosa cell; High-Throughput Nucleotide Sequencing; Humans; Leydig cell; Male; oocyte; ovary; Receptors, Notch - metabolism; Sertoli cell; Signal Transduction - physiology; single-cell RNA sequencing; Stem Cell Niche - physiology; testis; single-cell RNA sequencing; Sertoli cell; ovary; oocyte; germline; gonad; granulosa cell; testis; germ cell; Leydig cell

Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche cel...