Video analysis of ex vivo beating hearts during preservation on the TransMedics® organ care system

Reliable biomarkers for assessing the viability of the donor hearts undergoing perfusion remain elusive. A unique feature of normothermic perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintained in a beating state throughout the preservation period. We applied a v...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in cardiovascular medicine Vol. 10; p. 1216917
Main Authors Mendiola Pla, Michelle, Berrettoni, Silvia, Lee, Franklin H, Rozzi, Giacomo, Marrano, Federica, Gross, Ryan T, Evans, Amy, Wendell, David C, Lezberg, Paul, Burattini, Margherita, Paolo Lo Muzio, Francesco, Fassina, Lorenzo, Milano, Carmelo A, Bang, Marie-Louise, Bowles, Dawn E, Miragoli, Michele
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 20.06.2023
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Reliable biomarkers for assessing the viability of the donor hearts undergoing perfusion remain elusive. A unique feature of normothermic perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintained in a beating state throughout the preservation period. We applied a video algorithm for an assessment of cardiac kinematics, video kinematic evaluation (Vi.Ki.E.), to the donor hearts undergoing perfusion on the OCS™ to assess the feasibility of applying this algorithm in this setting. Healthy donor porcine hearts (  = 6) were procured from Yucatan pigs and underwent 2 h of normothermic perfusion on the OCS™ device. During the preservation period, serial high-resolution videos were captured at 30 frames per second. Using Vi.Ki.E., we assessed the force, energy, contractility, and trajectory parameters of each heart. There were no significant changes in any of the measured parameters of the heart on the OCS™ device over time as judged by linear regression analysis. Importantly, there were no significant changes in contractility during the duration of the preservation period (time 0-30 min, 918 ± 430 px/s; time 31-60 min, 1,386 ± 603 px/s; time 61-90 min, 1,299 ± 617 px/s; time 91-120 min, 1,535 ± 728 px/s). Similarly, there were no significant changes in the force, energy, or trajectory parameters. Post-transplantation echocardiograms demonstrated robust contractility of each allograft. Vi.Ki.E. assessment of the donor hearts undergoing perfusion is feasible on the TransMedics OCS™, and we observed that the donor hearts maintain steady kinematic measurements throughout the duration.
AbstractList BackgroundReliable biomarkers for assessing the viability of the donor hearts undergoing ex vivo perfusion remain elusive. A unique feature of normothermic ex vivo perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintained in a beating state throughout the preservation period. We applied a video algorithm for an in vivo assessment of cardiac kinematics, video kinematic evaluation (Vi.Ki.E.), to the donor hearts undergoing ex vivo perfusion on the OCS™ to assess the feasibility of applying this algorithm in this setting.MethodsHealthy donor porcine hearts (n = 6) were procured from Yucatan pigs and underwent 2 h of normothermic ex vivo perfusion on the OCS™ device. During the preservation period, serial high-resolution videos were captured at 30 frames per second. Using Vi.Ki.E., we assessed the force, energy, contractility, and trajectory parameters of each heart.ResultsThere were no significant changes in any of the measured parameters of the heart on the OCS™ device over time as judged by linear regression analysis. Importantly, there were no significant changes in contractility during the duration of the preservation period (time 0–30 min, 918 ± 430 px/s; time 31–60 min, 1,386 ± 603 px/s; time 61–90 min, 1,299 ± 617 px/s; time 91–120 min, 1,535 ± 728 px/s). Similarly, there were no significant changes in the force, energy, or trajectory parameters. Post-transplantation echocardiograms demonstrated robust contractility of each allograft.ConclusionVi.Ki.E. assessment of the donor hearts undergoing ex vivo perfusion is feasible on the TransMedics OCS™, and we observed that the donor hearts maintain steady kinematic measurements throughout the duration.
Reliable biomarkers for assessing the viability of the donor hearts undergoing perfusion remain elusive. A unique feature of normothermic perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintained in a beating state throughout the preservation period. We applied a video algorithm for an assessment of cardiac kinematics, video kinematic evaluation (Vi.Ki.E.), to the donor hearts undergoing perfusion on the OCS™ to assess the feasibility of applying this algorithm in this setting. Healthy donor porcine hearts (  = 6) were procured from Yucatan pigs and underwent 2 h of normothermic perfusion on the OCS™ device. During the preservation period, serial high-resolution videos were captured at 30 frames per second. Using Vi.Ki.E., we assessed the force, energy, contractility, and trajectory parameters of each heart. There were no significant changes in any of the measured parameters of the heart on the OCS™ device over time as judged by linear regression analysis. Importantly, there were no significant changes in contractility during the duration of the preservation period (time 0-30 min, 918 ± 430 px/s; time 31-60 min, 1,386 ± 603 px/s; time 61-90 min, 1,299 ± 617 px/s; time 91-120 min, 1,535 ± 728 px/s). Similarly, there were no significant changes in the force, energy, or trajectory parameters. Post-transplantation echocardiograms demonstrated robust contractility of each allograft. Vi.Ki.E. assessment of the donor hearts undergoing perfusion is feasible on the TransMedics OCS™, and we observed that the donor hearts maintain steady kinematic measurements throughout the duration.
Author Fassina, Lorenzo
Mendiola Pla, Michelle
Miragoli, Michele
Lezberg, Paul
Bowles, Dawn E
Evans, Amy
Burattini, Margherita
Marrano, Federica
Wendell, David C
Milano, Carmelo A
Paolo Lo Muzio, Francesco
Bang, Marie-Louise
Gross, Ryan T
Rozzi, Giacomo
Lee, Franklin H
Berrettoni, Silvia
AuthorAffiliation 4 Duke Cardiovascular Magnetic Resonance Center , Duke University Medical Center , Durham, NC , United States
1 Department of Surgery , Duke University Medical Center , Durham, NC , United States
7 Department of Electrical, Computer, and Biomedical Engineering , University of Pavia , Pavia , Italy
5 TransMedics, Inc. , Andover, MA , United States
3 Perfusion Services , Duke University Medical Center , Durham, NC , United States
8 Institute of Genetic and Biomedical Research (IRGB) , National Research Council (CNR ), Milan Unit , Milan , Italy
2 Department of Medicine and Surgery , University of Parma , Parma , Italy
9 IRCCS Humanitas Research Hospital , Milan , Italy
6 Department of Surgical Sciences, Dentistry, and Maternity , University of Verona , Verona , Italy
AuthorAffiliation_xml – name: 7 Department of Electrical, Computer, and Biomedical Engineering , University of Pavia , Pavia , Italy
– name: 4 Duke Cardiovascular Magnetic Resonance Center , Duke University Medical Center , Durham, NC , United States
– name: 2 Department of Medicine and Surgery , University of Parma , Parma , Italy
– name: 1 Department of Surgery , Duke University Medical Center , Durham, NC , United States
– name: 8 Institute of Genetic and Biomedical Research (IRGB) , National Research Council (CNR ), Milan Unit , Milan , Italy
– name: 3 Perfusion Services , Duke University Medical Center , Durham, NC , United States
– name: 6 Department of Surgical Sciences, Dentistry, and Maternity , University of Verona , Verona , Italy
– name: 5 TransMedics, Inc. , Andover, MA , United States
– name: 9 IRCCS Humanitas Research Hospital , Milan , Italy
Author_xml – sequence: 1
  givenname: Michelle
  surname: Mendiola Pla
  fullname: Mendiola Pla, Michelle
  organization: Department of Surgery, Duke University Medical Center, Durham, NC, United States
– sequence: 2
  givenname: Silvia
  surname: Berrettoni
  fullname: Berrettoni, Silvia
  organization: Department of Medicine and Surgery, University of Parma, Parma, Italy
– sequence: 3
  givenname: Franklin H
  surname: Lee
  fullname: Lee, Franklin H
  organization: Department of Surgery, Duke University Medical Center, Durham, NC, United States
– sequence: 4
  givenname: Giacomo
  surname: Rozzi
  fullname: Rozzi, Giacomo
  organization: Department of Medicine and Surgery, University of Parma, Parma, Italy
– sequence: 5
  givenname: Federica
  surname: Marrano
  fullname: Marrano, Federica
  organization: Department of Medicine and Surgery, University of Parma, Parma, Italy
– sequence: 6
  givenname: Ryan T
  surname: Gross
  fullname: Gross, Ryan T
  organization: Department of Surgery, Duke University Medical Center, Durham, NC, United States
– sequence: 7
  givenname: Amy
  surname: Evans
  fullname: Evans, Amy
  organization: Perfusion Services, Duke University Medical Center, Durham, NC, United States
– sequence: 8
  givenname: David C
  surname: Wendell
  fullname: Wendell, David C
  organization: Duke Cardiovascular Magnetic Resonance Center, Duke University Medical Center, Durham, NC, United States
– sequence: 9
  givenname: Paul
  surname: Lezberg
  fullname: Lezberg, Paul
  organization: TransMedics, Inc., Andover, MA, United States
– sequence: 10
  givenname: Margherita
  surname: Burattini
  fullname: Burattini, Margherita
  organization: Department of Surgical Sciences, Dentistry, and Maternity, University of Verona, Verona, Italy
– sequence: 11
  givenname: Francesco
  surname: Paolo Lo Muzio
  fullname: Paolo Lo Muzio, Francesco
  organization: Department of Medicine and Surgery, University of Parma, Parma, Italy
– sequence: 12
  givenname: Lorenzo
  surname: Fassina
  fullname: Fassina, Lorenzo
  organization: Department of Electrical, Computer, and Biomedical Engineering, University of Pavia, Pavia, Italy
– sequence: 13
  givenname: Carmelo A
  surname: Milano
  fullname: Milano, Carmelo A
  organization: Department of Surgery, Duke University Medical Center, Durham, NC, United States
– sequence: 14
  givenname: Marie-Louise
  surname: Bang
  fullname: Bang, Marie-Louise
  organization: IRCCS Humanitas Research Hospital, Milan, Italy
– sequence: 15
  givenname: Dawn E
  surname: Bowles
  fullname: Bowles, Dawn E
  organization: Department of Surgery, Duke University Medical Center, Durham, NC, United States
– sequence: 16
  givenname: Michele
  surname: Miragoli
  fullname: Miragoli, Michele
  organization: IRCCS Humanitas Research Hospital, Milan, Italy
BackLink https://www.ncbi.nlm.nih.gov/pubmed/37408655$$D View this record in MEDLINE/PubMed
BookMark eNpVkc1uEzEQgC1UREvpA3BBPnJJ8N_a3hNCVSmVirgUxM3yzzjZatcO9mZFXoqH4Mm6IaFqJUv2eGY-j_W9RicpJ0DoLSVLznX7IfppWDLC-JIyKluqXqAzxlq1IE3z8-TJ-RRd1HpPCKGN0I3Ur9ApV4Jo2TRnyP_oAmRsk-13tas4Rwy_8dRNGTuwY5dWeA22jBWHbdlHmwIVyjSncsLzGteA74pN9SuEzte_f3AuK5uwtwVw3dURhjfoZbR9hYvjfo6-f766u_yyuP12fXP56XbhhZTjQikuXKsIk0w64qPVLrRaSscDd14riCzSGKTkIIKjKnrHQQdBGFhOeOTn6ObADdnem03pBlt2JtvO_LuY5zLzTzrfg7EEGCVCadYEEaNyzCmmuHKOSuUimVkfD6zN1g0QPKSx2P4Z9HkmdWuzypOhhFPNm3YmvD8SSv61hTqaoase-t4myNtqmOa8bbVgYi6lh1Jfcq0F4uM7lJi9bLOXbfayzVH23PPu6YCPHf_V8gdW1qq7
CitedBy_id crossref_primary_10_1038_s41598_024_52081_9
crossref_primary_10_1016_j_device_2023_100217
Cites_doi 10.3389/ti.2022.10258
10.3390/jcm10225330
10.1016/j.healun.2022.02.003
10.1038/srep46143
10.1111/ajt.12994
10.1097/TP.0000000000004167
10.1093/icvts/ivz120
10.1016/j.xjon.2021.05.010
10.1016/j.healun.2019.01.088
10.1016/j.jhep.2014.04.019
10.3389/fmed.2021.808719
10.1093/ejcts/ezab026
10.3791/63114
ContentType Journal Article
Copyright 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli.
2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli. 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli
Copyright_xml – notice: 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli.
– notice: 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli. 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli
DBID NPM
AAYXX
CITATION
7X8
5PM
DOA
DOI 10.3389/fcvm.2023.1216917
DatabaseName PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle PubMed
CrossRef
MEDLINE - Academic
DatabaseTitleList
MEDLINE - Academic
PubMed
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 2297-055X
EndPage 1216917
ExternalDocumentID oai_doaj_org_article_a0e21047825d4ff7b2b72737bb167bf0
10_3389_fcvm_2023_1216917
37408655
Genre Journal Article
GrantInformation_xml – fundername: European Union–NextGenerationEU
  grantid: ECS00000033
– fundername: National Recovery and Resilience Plan (NRRP)
  grantid: 3277
– fundername: Duke Division of Cardiothoracic Surgery and TransMedics, Inc.
  grantid: T32HL007101
– fundername: ERA PerMed
  grantid: ERAPERMED2018-271
GroupedDBID 53G
5VS
9T4
AAFWJ
ACGFS
ACXDI
ADBBV
ADRAZ
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BCNDV
GROUPED_DOAJ
HYE
IAO
IEA
IHR
IHW
IPNFZ
KQ8
M48
M~E
NPM
OK1
PGMZT
RIG
RPM
AAYXX
CITATION
7X8
5PM
AFPKN
ID FETCH-LOGICAL-c466t-7734b9702626b0cfa8bd9866b3d3bc87ef2f1fd663e4db17fcb3e8d402ea303f3
IEDL.DBID RPM
ISSN 2297-055X
IngestDate Tue Oct 22 14:37:55 EDT 2024
Tue Sep 17 21:30:29 EDT 2024
Sat Oct 05 06:08:51 EDT 2024
Thu Nov 21 23:43:24 EST 2024
Wed Oct 16 00:37:49 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Keywords kinematics
normothermic
video
biomarker
ex vivo perfusion
cardiac transplantation
Language English
License 2023 Mendiola Pla, Berrettoni, Lee, Rozzi, Marrano, Gross, Evans, Wendell, Lezberg, Burattini, Paolo lo Muzio, Fassina, Milano, Bang, Bowles and Miragoli.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c466t-7734b9702626b0cfa8bd9866b3d3bc87ef2f1fd663e4db17fcb3e8d402ea303f3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors share first authorship
Edited by: Matthias Loebe, Jackson Health System, United States
Reviewed by: Charles Hoopes, University of Alabama at Birmingham, United StatesStephen Ralph Large, Papworth Hospital, United Kingdom
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10318359/
PMID 37408655
PQID 2833998424
PQPubID 23479
PageCount 1
ParticipantIDs doaj_primary_oai_doaj_org_article_a0e21047825d4ff7b2b72737bb167bf0
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10318359
proquest_miscellaneous_2833998424
crossref_primary_10_3389_fcvm_2023_1216917
pubmed_primary_37408655
PublicationCentury 2000
PublicationDate 2023-06-20
PublicationDateYYYYMMDD 2023-06-20
PublicationDate_xml – month: 06
  year: 2023
  text: 2023-06-20
  day: 20
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
PublicationTitle Frontiers in cardiovascular medicine
PublicationTitleAlternate Front Cardiovasc Med
PublicationYear 2023
Publisher Frontiers Media S.A
Publisher_xml – name: Frontiers Media S.A
References Graham (B1) 2014; 61
Langmuur (B5) 2022; 106
Qin (B3) 2022; 35
Fassina (B8) 2017; 7
Hull (B12) 2021; 8
Mendiola Pla (B11) 2022; 180
Lo Muzio (B13) 2021; 10
Rozzi (B10) 2021; 59
Schroder (B4) 2019; 38
Zulpaite (B2) 2021; 8
Cernic (B6) 2022; 41
Rozzi (B9) 2019; 29
Iyer (B7) 2015; 15
References_xml – volume: 35
  start-page: 10258
  year: 2022
  ident: B3
  article-title: Machine perfusion for human heart preservation: a systematic review
  publication-title: Transpl Int
  doi: 10.3389/ti.2022.10258
  contributor:
    fullname: Qin
– volume: 10
  start-page: 5330
  year: 2021
  ident: B13
  article-title: Artificial intelligence supports decision making during open-chest surgery of rare congenital heart defects
  publication-title: J Clin Med
  doi: 10.3390/jcm10225330
  contributor:
    fullname: Lo Muzio
– volume: 41
  start-page: 1294
  year: 2022
  ident: B6
  article-title: Lactate during ex-situ heart perfusion does not predict the requirement for mechanical circulatory support following donation after circulatory death (DCD) heart transplants
  publication-title: J Heart Lung Transplant
  doi: 10.1016/j.healun.2022.02.003
  contributor:
    fullname: Cernic
– volume: 7
  start-page: 46143
  year: 2017
  ident: B8
  article-title: Cardiac kinematic parameters computed from video of in situ beating heart
  publication-title: Sci Rep
  doi: 10.1038/srep46143
  contributor:
    fullname: Fassina
– volume: 15
  start-page: 371
  year: 2015
  ident: B7
  article-title: Normothermic ex vivo perfusion provides superior organ preservation and enables viability assessment of hearts from DCD donors
  publication-title: Am J Transplant
  doi: 10.1111/ajt.12994
  contributor:
    fullname: Iyer
– volume: 106
  start-page: 1745
  year: 2022
  ident: B5
  article-title: Normothermic ex situ heart perfusion with the organ care system for cardiac transplantation: a meta-analysis
  publication-title: Transplantation
  doi: 10.1097/TP.0000000000004167
  contributor:
    fullname: Langmuur
– volume: 29
  start-page: 625
  year: 2019
  ident: B9
  article-title: Real-time video kinematic evaluation of the in situ beating right ventricle after pulmonary valve replacement in patients with tetralogy of Fallot: a pilot study
  publication-title: Interact Cardiovasc Thorac Surg
  doi: 10.1093/icvts/ivz120
  contributor:
    fullname: Rozzi
– volume: 8
  start-page: 128
  year: 2021
  ident: B12
  article-title: Primary graft dysfunction in heart transplantation: how to recognize it, when to institute extracorporeal membrane oxygenation, and outcomes
  publication-title: JTCVS Open
  doi: 10.1016/j.xjon.2021.05.010
  contributor:
    fullname: Hull
– volume: 38
  start-page: S42
  year: 2019
  ident: B4
  article-title: Successful utilization of extended criteria donor (ECD) hearts for transplantation—results of the OCS™ heart EXPAND trial to evaluate the effectiveness and safety of the OCS heart system to preserve and assess ECD hearts for transplantation
  publication-title: J Heart Lung Transplant
  doi: 10.1016/j.healun.2019.01.088
  contributor:
    fullname: Schroder
– volume: 61
  start-page: 418
  year: 2014
  ident: B1
  article-title: “Resuscitation” of marginal liver allografts for transplantation with machine perfusion technology
  publication-title: J Hepatol
  doi: 10.1016/j.jhep.2014.04.019
  contributor:
    fullname: Graham
– volume: 8
  start-page: 808719
  year: 2021
  ident: B2
  article-title: Ex-vivo kidney machine perfusion: therapeutic potential
  publication-title: Front Med
  doi: 10.3389/fmed.2021.808719
  contributor:
    fullname: Zulpaite
– volume: 59
  start-page: 1329
  year: 2021
  ident: B10
  article-title: Right ventricular functional recovery depends on timing of pulmonary valve replacement in tetralogy of Fallot: a video kinematic study
  publication-title: Eur J Cardiothorac Surg
  doi: 10.1093/ejcts/ezab026
  contributor:
    fullname: Rozzi
– volume: 180
  start-page: e63114
  year: 2022
  ident: B11
  article-title: A porcine heterotopic heart transplantation protocol for delivery of therapeutics to a cardiac allograft
  publication-title: J Vis Exp
  doi: 10.3791/63114
  contributor:
    fullname: Mendiola Pla
SSID ssj0001548568
Score 2.296033
Snippet Reliable biomarkers for assessing the viability of the donor hearts undergoing perfusion remain elusive. A unique feature of normothermic perfusion on the...
BackgroundReliable biomarkers for assessing the viability of the donor hearts undergoing ex vivo perfusion remain elusive. A unique feature of normothermic ex...
SourceID doaj
pubmedcentral
proquest
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 1216917
SubjectTerms biomarker
cardiac transplantation
Cardiovascular Medicine
ex vivo perfusion
kinematics
normothermic
video
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwELVQD4gLonwGKDISJ6Soie21nWNBrSokOFHUm-XxB-wlQex2xa_iR_SXMWNnyy6qxAUppySKnTdJ5k0884axN2AMsdDcIhn2LbncFpDFtjZAQPagpK3ZFp_0-YX6cLm43Gn1RTlhVR64AnfsuySKhIxYRJWzAQHkcg1Arw3kGq13YieYqvXByi60rcuYGIUNxzlsqPBcSNJT0ENpUPbHERW9_ttI5t-5kjvO5-wBuz-zRn5SZ3vI7qTxIbv7cV4Xf8TCl2VME_ezwgifMk8_-Wa5mTgQKRy_cupcvV7xWpbIKf11-zuW44Y0kBe3Va65uv7FS7snTolhvKo9P2YXZ6ef35-3c_uENiit18ibpYLBYJAlNHQhewtxsFqDjBKCNSmL3OeIlCOpCL3JAWSyEQPK5NGxZfmEHYzTmJ4xHKyTg01R9T4qkYX3Jnc5hhBUgChzw95usXTfq0qGw-iCgHcEvCPg3Qx8w94R2jcnksB12YE35mazu3-ZvWGvt7Zy-ELQKocf03S1csiXkHRZJVTDnlbb3QwljeqoErdhds-qe3PZPzIuvxXR7b58_RbD8_8x-xfsHiFCKWeie8kO1j-u0hGSmzW8Ks_xb_vj-jY
  priority: 102
  providerName: Directory of Open Access Journals
Title Video analysis of ex vivo beating hearts during preservation on the TransMedics® organ care system
URI https://www.ncbi.nlm.nih.gov/pubmed/37408655
https://search.proquest.com/docview/2833998424
https://pubmed.ncbi.nlm.nih.gov/PMC10318359
https://doaj.org/article/a0e21047825d4ff7b2b72737bb167bf0
Volume 10
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELbaHhAXRHkGSmUkTkjpJrbXdo5tRVUhFXGgqLfI4wesRJOK3a74Vf0R_WXMOEnpIk5IOeVlZ2ai-cb-Zoaxd2AModBUIhh2JbncEhDFltaDR_SgpB3YFp_06bn6eDG_2GJ6yoXJpH0Pi4Pux-VBt_ieuZVXl3428cRmn8-O62yJ82a2zbbR_96L0YfcYGXn2g5bmBiBNbPk15R0LiTVUtBNbk72xwnlWv3_Aph_8yTvOZ6Tx-zRiBj54TCzXbYVuyfswdm4J_6U-a-LEHvuxuoivE88_uLrxbrnQICw-8apa_VqyYeURE7U12kpluOBEJBnl5Xfuby94bnVEydSGB8qPT9j5ycfvhyflmPrhNIrrVeImaWCxmCAJTRUPjkLobFagwwSvDUxiVSngHAjqgC1SR5ktAGDyejQqSX5nO10fRdfMhysko2NQdUuKJGEcyZVKXjvlYcgU8HeT7Jsr4YKGS1GFiT4lgTfkuDbUfAFOyJp391Ixa3zCfywdlRx66ooctUgMQ8qJQMCCGUZgFobSFXB3k66avFnoB0O18X-etkiVkLAZZVQBXsx6O5uKGlURVm4BbMbWt2Yy-YVtL9ccHuyt1f__-hr9pDkQCQzUe2xndXP6_gG4cwK9vMywH624d9J-vqx
link.rule.ids 230,314,727,780,784,864,885,2102,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELZKkaAXVN6BAkbihJRuYju2c6RVqwW6FYcW9WZl_Cgr0aTqblf8Kn4Ev4yxk5Qu4oSUU57OzETzTfzNZ0LegVIRhYYcwXCTx5SbA6LYXFuwiB4E1z3b4lhOT8Wns-psg8ixFyaR9i3Md9vvF7vt_FviVl5e2MnIE5t8me2XKRKrenKH3K24qstbVXrfHSx0JXU_iYk1WD0JdhXbzhmPagqyTsuT_UlDSa3_XxDzb6bkrdRzuE0eDJiRfujH9pBs-PYRuTcbZsUfE_t17nxHm0FfhHaB-h90NV91FCIkbM9pXLd6uaB9UyKN5NfxZyzFDUEgTUkr3XPx6ydNiz3RSAujvdbzE3J6eHCyP82HxRNyK6RcImrmAmqFJRaTUNjQaHC1lhK442C18oGFMjgEHF44KFWwwL12WE76BtNa4E_JZtu1_jnBhxW81t6JsnGCBdY0KhTBWWuFBcdDRt6PtjSXvUaGwdoiGt5Ew5toeDMYPiN70do3J0Z567QDX8wMTjZN4VnSDWKVEyEoYBBxlgIopYJQZOTt6CuDn0Oc42ha310vDKIlhFxaMJGRZ73vbh7FlShiH25G9JpX18ayfgQjMElujxH34v8vfUPuT09mR-bo4_Hnl2Qr2iRSzlixQzaXV9f-FYKbJbxOkfwbreH9EQ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELagSBUXRHk1QMFInJDSJLZjO0corMqjVQ8U9WZl_ICV2mTFblf8Kn4Ev4yxk5Qu4oSUU57OzETzTfzNZ0JeglIRhYYcwXCbx5SbA6LYXFuwiB4E1wPb4lgenooPZ_XZyKpcjrTKzsJ8vzu_2O_m3xK3cnFhi4knVpwcHVQpEuumWLhQ3CS3ao5Rdq1SHzqEha6lHiYysQ5rimDXsfWc8aioIJu0RNmfVJQU-_8FM_9mS15LP7O75M6IG-nrYXw75Ibv7pHto3Fm_D6xX-bO97QdNUZoH6j_QdfzdU8hwsLuK41rV6-WdGhMpJEAO_2QpbghEKQpcaV7Ln_9pGnBJxqpYXTQe35ATmfvPh8c5uMCCrkVUq4QOXMBjcIyi0kobWg1uEZLCdxxsFr5wEIVHIIOLxxUKljgXjssKX2LqS3wh2Sr6zu_S_BhJW-0d6JqnWCBta0KZXDWWmHB8ZCRV5MtzWLQyTBYX0TDm2h4Ew1vRsNn5E209tWJUeI67cAXM6OjTVt6lrSDWO1ECAoYRKylACqpIJQZeTH5yuAnEec52s73l0uDiAlhlxZMZOTR4LurR3ElytiLmxG94dWNsWwewShMsttT1D3-_0ufk-2TtzPz6f3xxyfkdjRJZJ2x8inZWn2_9HuIb1bwLAXybzqh_iQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Video+analysis+of+ex+vivo+beating+hearts+during+preservation+on+the+TransMedics%C2%AE+organ+care+system&rft.jtitle=Frontiers+in+cardiovascular+medicine&rft.au=Michelle+Mendiola+Pla&rft.au=Silvia+Berrettoni&rft.au=Franklin+H.+Lee&rft.au=Giacomo+Rozzi&rft.date=2023-06-20&rft.pub=Frontiers+Media+S.A&rft.eissn=2297-055X&rft.volume=10&rft_id=info:doi/10.3389%2Ffcvm.2023.1216917&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_a0e21047825d4ff7b2b72737bb167bf0
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2297-055X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2297-055X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2297-055X&client=summon