Liraglutide attenuates obese-associated breast cancer cell proliferation via inhibiting PI3K/Akt/mTOR signaling pathway

• Published in: Saudi pharmaceutical journal Vol. 32; no. 1; p. 101923
• Main Authors: Alanteet, Alaa, Attia, Hala, Alfayez, Musaed, Mahmood, Amer, Alsaleh, Khalid, Alsanea, Sary
• Format: Journal Article
• Language: English
• Published: Saudi Arabia Elsevier B.V 01.01.2024; Springer
• Subjects: Akt; Breast Cancer; Liraglutide; mTOR; Obesity; PI3K; PBS; Obesity; BC; CM; siRNA; Akt; ADSCs-CM; Breast Cancer; SC; BSA; DMEM; SIRT-1; p-Akt; PI3K; ADSCs; FBS; mTOR; LGT; AMPK; Liraglutide; BMI; GLP-1R
• ISSN: 1319-0164
• DOI: 10.1016/j.jsps.2023.101923

This study aims to explore the anti-proliferative, pro-apoptotic, and anti-migration activities of liraglutide (LGT) in MCF-7 breast cancer (BC) cells in subjects with obesity, particularly its effects on the PI3K/Akt/mTOR/AMPK pathway. The role of AMPK/SIRT-1, an essential regulator of adipokine production, in the effect of LGT on the production of adipose-derived adipokine was also assessed. MCF-7 cells were incubated in conditioned medium (CM) generated from adipose-derived stem cells (ADSCs) of obese subjects. MCF-7 cells were then treated with LGT for 72 h. Anti-proliferative, pro-apoptotic, and anti-migration activities were investigated using alamarBlue, annexin V stain, and scratch assay, respectively. Protein levels of phosphorylated PI3K, p-Akt, p-mTOR, and p-AMPK were investigated using immunoblotting. Levels of adipokines in ADSCs were determined using RT-PCR before and after transfection of ADSCs using the specific small interference RNA sequences for AMPK and SIRT-1. LGT evoked anti-proliferative, apoptotic, and potential anti-migratory properties on MCF-7 cells incubated in CM from obese ADSCs and significantly mitigated the activity of the PI3K/Akt/mTOR survival pathway—but not AMPK—in MCF-7 cells. Furthermore, the anti-proliferative effects afforded by LGT were similar to those mediated by LY294002 (PI3K inhibitor) and rapamycin (mTOR inhibitor). Our results reveal that transfection of AMPK/SIRT-1 genes did not affect the beneficial role of LGT in the expression of adipokines in ADSCs. In conclusion, LGT elicits anti-proliferative, apoptotic, and anti-migratory effects on BC cells in obese conditions by suppressing the activity of survival pathways; however, this effect is independent of the AMPK/SIRT1 pathway in ADSCs or AMPK in BC cells.