Inhibition of protein tyrosine phosphatases unmasks vasoconstriction and potentiates calcium signaling in rat aorta smooth muscle cells in response to an agonist of 5-HT2B receptors BW723C86
In blood vessels, serotonin 5-HT2B receptors mainly mediate relaxation, although their activation by the selective agonist BW723C86 is known to exert contraction of aorta in deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-l-arginine (l-NAME) hypertensive rats [Russel et al., 2002; Banes e...
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Published in | Biochemical and biophysical research communications Vol. 483; no. 1; pp. 700 - 705 |
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29.01.2017
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Abstract | In blood vessels, serotonin 5-HT2B receptors mainly mediate relaxation, although their activation by the selective agonist BW723C86 is known to exert contraction of aorta in deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-l-arginine (l-NAME) hypertensive rats [Russel et al., 2002; Banes et al., 2003] and in mice with type 2 diabetes [Nelson et al., 2012]. The unmasking effect on vasoconstriction can be caused by a shift in the balance of tyrosine phosphorylation in smooth muscle cells (SMC) due to oxidative stress induced inhibition of protein tyrosine phosphatases (PTP). We have demonstrated that BW723C86 which does not cause contraction of rat aorta and mesenteric artery rings, evoked a vasoconstrictor effect in the presence of PTP inhibitors sodium orthovanadate (Na3VO4) or BVT948. BW723C86 induced a weak rise of [Ca2+]i in the SMC isolated from rat aorta; however, after pre-incubation with Na3VO4 the response to BW723C86 increased more than 5-fold. This effect was diminished by protein tyrosine kinase (PTK) inhibitor genistein, inhibitor of Src-family kinases PP2, inhibitor of NADPH-oxidase VAS2870 and completely suppressed by N-acetylcysteine and 5-HT2B receptor antagonist RS127445. Using fluorescent probe DCFH-DA we have shown that Na3VO4 induces oxidative stress in SMC. In the presence of Na3VO4 BW723C86 considerably increased formation of reactive oxygen species while alone had no appreciable effect on DCFH oxidation. We suggest that oxidative stress causes inhibition of PTP and unmasking of 5-HT2B receptors functional activity. |
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AbstractList | In blood vessels, serotonin 5-HT2B receptors mainly mediate relaxation, although their activation by the selective agonist BW723C86 is known to exert contraction of aorta in deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-l-arginine (l-NAME) hypertensive rats [Russel et al., 2002; Banes et al., 2003] and in mice with type 2 diabetes [Nelson et al., 2012]. The unmasking effect on vasoconstriction can be caused by a shift in the balance of tyrosine phosphorylation in smooth muscle cells (SMC) due to oxidative stress induced inhibition of protein tyrosine phosphatases (PTP). We have demonstrated that BW723C86 which does not cause contraction of rat aorta and mesenteric artery rings, evoked a vasoconstrictor effect in the presence of PTP inhibitors sodium orthovanadate (Na3VO4) or BVT948. BW723C86 induced a weak rise of [Ca2+]i in the SMC isolated from rat aorta; however, after pre-incubation with Na3VO4 the response to BW723C86 increased more than 5-fold. This effect was diminished by protein tyrosine kinase (PTK) inhibitor genistein, inhibitor of Src-family kinases PP2, inhibitor of NADPH-oxidase VAS2870 and completely suppressed by N-acetylcysteine and 5-HT2B receptor antagonist RS127445. Using fluorescent probe DCFH-DA we have shown that Na3VO4 induces oxidative stress in SMC. In the presence of Na3VO4 BW723C86 considerably increased formation of reactive oxygen species while alone had no appreciable effect on DCFH oxidation. We suggest that oxidative stress causes inhibition of PTP and unmasking of 5-HT2B receptors functional activity. In blood vessels, serotonin 5-HT2B receptors mainly mediate relaxation, although their activation by the selective agonist BW723C86 is known to exert contraction of aorta in deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-l-arginine (l-NAME) hypertensive rats [Russel et al., 2002; Banes et al., 2003] and in mice with type 2 diabetes [Nelson et al., 2012]. The unmasking effect on vasoconstriction can be caused by a shift in the balance of tyrosine phosphorylation in smooth muscle cells (SMC) due to oxidative stress induced inhibition of protein tyrosine phosphatases (PTP). We have demonstrated that BW723C86 which does not cause contraction of rat aorta and mesenteric artery rings, evoked a vasoconstrictor effect in the presence of PTP inhibitors sodium orthovanadate (Na VO ) or BVT948. BW723C86 induced a weak rise of [Ca ] in the SMC isolated from rat aorta; however, after pre-incubation with Na VO the response to BW723C86 increased more than 5-fold. This effect was diminished by protein tyrosine kinase (PTK) inhibitor genistein, inhibitor of Src-family kinases PP2, inhibitor of NADPH-oxidase VAS2870 and completely suppressed by N-acetylcysteine and 5-HT2B receptor antagonist RS127445. Using fluorescent probe DCFH-DA we have shown that Na VO induces oxidative stress in SMC. In the presence of Na VO BW723C86 considerably increased formation of reactive oxygen species while alone had no appreciable effect on DCFH oxidation. We suggest that oxidative stress causes inhibition of PTP and unmasking of 5-HT2B receptors functional activity. |
Author | Mironova, Galina Y. Goncharov, Nikolay V. Jenkins, Richard O. Avdonin, Pavel V. Avdonin, Piotr P. |
Author_xml | – sequence: 1 givenname: Galina Y. surname: Mironova fullname: Mironova, Galina Y. organization: Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia – sequence: 2 givenname: Piotr P. surname: Avdonin fullname: Avdonin, Piotr P. organization: Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia – sequence: 3 givenname: Nikolay V. surname: Goncharov fullname: Goncharov, Nikolay V. organization: Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia – sequence: 4 givenname: Richard O. surname: Jenkins fullname: Jenkins, Richard O. organization: School of Allied Health Sciences, De Montfort University, Leicester, UK – sequence: 5 givenname: Pavel V. surname: Avdonin fullname: Avdonin, Pavel V. email: pvavdonin@yandex.ru organization: Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia |
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Cites_doi | 10.1124/pr.111.004697 10.1007/s10616-009-9236-6 10.1139/y03-108 10.1124/jpet.102.037390 10.1007/978-1-60761-411-1_4 10.1161/hy02t2.102793 10.5483/BMBRep.2013.46.12.103 10.1016/j.addr.2007.03.005 10.1016/S0891-5849(98)00162-2 10.1016/0014-5793(95)00828-W 10.1186/1471-2210-3-12 10.1016/j.freeradbiomed.2008.08.013 10.1097/00005344-200110000-00006 10.1016/j.ntt.2010.10.010 10.1016/j.vph.2012.02.012 10.1124/jpet.103.062745 10.1006/abbi.1993.1366 10.1126/science.1130481 10.1023/A:1006818810678 10.1097/00004872-200103000-00012 10.1081/CEH-200031904 10.1155/2012/398406 10.1113/jphysiol.2008.153593 10.1124/jpet.103.062562 10.1007/s00018-012-1107-1 10.1038/sj.bjp.0702751 10.1074/jbc.272.2.843 |
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Keywords | Hypertension Oxidative stress Protein tyrosine phosphatases Calcium Smooth muscle cells 5-HT2B receptors |
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References | Watts, Yang, Banes, Baez (bib1) 2001; 38 Chang, Park, Lee (bib27) 2013; 46 Eruslanov, Kusmartsev (bib19) 2010; 594 Srivastava, St-Louis (bib24) 1997; 176 Russell, Banes, Berlin, Fink, Watts (bib8) 2002; 303 Xu, Fu, Chen, Xiao, Lan, Le, Cheng, He, Shen, Huang, Liu (bib14) 2009; 61 Di Salvo, Semenchuk, Lauer (bib23) 1993; 304 Watts, Morrison, Davis, Barman (bib3) 2012; 64 Wingler, Altenhoefer, Kleikers, Radermacher, Kleinschnitz, Schmidt (bib21) 2012; 69 Lu, Alioua, Kumar, Kundu, Eghbali, Weisstaub, Gingrich, Stefani, Toro (bib10) 2008; 586 Ullmer, Schmuck, Kalkman, Lubbert (bib2) 1995; 370 Aagaard, Rossi (bib18) 2007; 59 Ishida, Kawashima, Hirata, Yokoyama (bib4) 1998; 44 Rhee (bib17) 2006; 312 Nelson, Harrod, Lamping (bib9) 2012; 2012 Porter, Benwell, Lamb, Malcolm, Allen, Revell, Adams, Sheardown (bib5) 1999; 128 Banes, Watts (bib29) 2002; 39 Offer, Mohsen, Samuni (bib20) 1998; 25 Saini, Sharma, Zahradka, Kumamoto, Takeda, Dhalla (bib15) 2003; 81 Liljebris, Baranczewski, Bjorkstrand, Bystrom, Lundgren, Tjernberg, Warolen, James (bib12) 2004; 309 Watts, Thompson (bib6) 2004; 309 Banes, Watts (bib7) 2003; 3 Cuesta, Frances, Garcia (bib16) 2011; 33 de Champlain, Wu, Girouard, Karas, E.L.M. A, Laplante, Wu (bib11) 2004; 26 Schramm, Matusik, Osmenda, Guzik (bib22) 2012; 56 Dikalov, Dikalova, Bikineyeva, Schmidt, Harrison, Griendling (bib26) 2008; 45 Huyer, Liu, Kelly, Moffat, Payette, Kennedy, Tsaprailis, Gresser, Ramachandran (bib13) 1997; 272 Touyz, Wu, He, Park, Chen, Vacher, Rajapurohitam, Schiffrin (bib25) 2001; 19 Watts, Baez, Webb (bib28) 1996; 277 Watts (10.1016/j.bbrc.2016.12.079_bib1) 2001; 38 Xu (10.1016/j.bbrc.2016.12.079_bib14) 2009; 61 Chang (10.1016/j.bbrc.2016.12.079_bib27) 2013; 46 Russell (10.1016/j.bbrc.2016.12.079_bib8) 2002; 303 Touyz (10.1016/j.bbrc.2016.12.079_bib25) 2001; 19 Ullmer (10.1016/j.bbrc.2016.12.079_bib2) 1995; 370 Saini (10.1016/j.bbrc.2016.12.079_bib15) 2003; 81 Nelson (10.1016/j.bbrc.2016.12.079_bib9) 2012; 2012 de Champlain (10.1016/j.bbrc.2016.12.079_bib11) 2004; 26 Watts (10.1016/j.bbrc.2016.12.079_bib3) 2012; 64 Offer (10.1016/j.bbrc.2016.12.079_bib20) 1998; 25 Banes (10.1016/j.bbrc.2016.12.079_bib7) 2003; 3 Aagaard (10.1016/j.bbrc.2016.12.079_bib18) 2007; 59 Porter (10.1016/j.bbrc.2016.12.079_bib5) 1999; 128 Cuesta (10.1016/j.bbrc.2016.12.079_bib16) 2011; 33 Watts (10.1016/j.bbrc.2016.12.079_bib6) 2004; 309 Rhee (10.1016/j.bbrc.2016.12.079_bib17) 2006; 312 Schramm (10.1016/j.bbrc.2016.12.079_bib22) 2012; 56 Huyer (10.1016/j.bbrc.2016.12.079_bib13) 1997; 272 Wingler (10.1016/j.bbrc.2016.12.079_bib21) 2012; 69 Lu (10.1016/j.bbrc.2016.12.079_bib10) 2008; 586 Dikalov (10.1016/j.bbrc.2016.12.079_bib26) 2008; 45 Di Salvo (10.1016/j.bbrc.2016.12.079_bib23) 1993; 304 Eruslanov (10.1016/j.bbrc.2016.12.079_bib19) 2010; 594 Banes (10.1016/j.bbrc.2016.12.079_bib29) 2002; 39 Srivastava (10.1016/j.bbrc.2016.12.079_bib24) 1997; 176 Watts (10.1016/j.bbrc.2016.12.079_bib28) 1996; 277 Ishida (10.1016/j.bbrc.2016.12.079_bib4) 1998; 44 Liljebris (10.1016/j.bbrc.2016.12.079_bib12) 2004; 309 |
References_xml | – volume: 309 start-page: 165 year: 2004 end-page: 172 ident: bib6 article-title: Characterization of the contractile 5-hydroxytryptamine receptor in the renal artery of the normotensive rat publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Thompson – volume: 69 start-page: 3159 year: 2012 end-page: 3160 ident: bib21 article-title: VAS2870 is a pan-NADPH oxidase inhibitor publication-title: Cell Mol. Life Sci. contributor: fullname: Schmidt – volume: 594 start-page: 57 year: 2010 end-page: 72 ident: bib19 article-title: Identification of ROS using oxidized DCFDA and flow-cytometry publication-title: Methods Mol. Biol. contributor: fullname: Kusmartsev – volume: 39 start-page: 394 year: 2002 end-page: 398 ident: bib29 article-title: Upregulation of arterial serotonin 1B and 2B receptors in deoxycorticosterone acetate-salt hypertension publication-title: Hypertension contributor: fullname: Watts – volume: 61 start-page: 65 year: 2009 end-page: 72 ident: bib14 article-title: Development of an optimized protocol for primary culture of smooth muscle cells from rat thoracic aortas publication-title: Cytotechnology contributor: fullname: Liu – volume: 64 start-page: 359 year: 2012 end-page: 388 ident: bib3 article-title: Serotonin and blood pressure regulation publication-title: Pharmacol. Rev. contributor: fullname: Barman – volume: 56 start-page: 216 year: 2012 end-page: 231 ident: bib22 article-title: Targeting NADPH oxidases in vascular pharmacology publication-title: Vasc. Pharmacol. contributor: fullname: Guzik – volume: 46 start-page: 600 year: 2013 end-page: 605 ident: bib27 article-title: Feasibility of simultaneous measurement of cytosolic calcium and hydrogen peroxide in vascular smooth muscle cells publication-title: BMB Rep. contributor: fullname: Lee – volume: 33 start-page: 297 year: 2011 end-page: 302 ident: bib16 article-title: ROS formation and antioxidant status in brain areas of rats exposed to sodium metavanadate publication-title: Neurotoxicol. Teratol. contributor: fullname: Garcia – volume: 19 start-page: 441 year: 2001 end-page: 449 ident: bib25 article-title: Role of c-Src in the regulation of vascular contraction and Ca publication-title: J. Hypertens. contributor: fullname: Schiffrin – volume: 128 start-page: 13 year: 1999 end-page: 20 ident: bib5 article-title: Functional characterization of agonists at recombinant human 5-HT2A, 5-HT2B and 5-HT2C receptors in CHO-K1 cells publication-title: Br. J. Pharmacol. contributor: fullname: Sheardown – volume: 277 start-page: 1103 year: 1996 end-page: 1113 ident: bib28 article-title: The 5-hydroxytryptamine2B receptor and 5-HT receptor signal transduction in mesenteric arteries from deoxycorticosterone acetate-salt hypertensive rats publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Webb – volume: 309 start-page: 711 year: 2004 end-page: 719 ident: bib12 article-title: Oxidation of protein tyrosine phosphatases as a pharmaceutical mechanism of action: a study using 4-hydroxy-3,3-dimethyl-2H-benzo[g]indole-2,5(3H)-dione publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: James – volume: 272 start-page: 843 year: 1997 end-page: 851 ident: bib13 article-title: Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate publication-title: J. Biol. Chem. contributor: fullname: Ramachandran – volume: 26 start-page: 593 year: 2004 end-page: 601 ident: bib11 article-title: Oxidative stress in hypertension publication-title: Clin. Exp. Hypertens. contributor: fullname: Wu – volume: 59 start-page: 75 year: 2007 end-page: 86 ident: bib18 article-title: RNAi therapeutics: principles, prospects and challenges publication-title: Adv. Drug Deliv. Rev. contributor: fullname: Rossi – volume: 38 start-page: 539 year: 2001 end-page: 551 ident: bib1 article-title: Activation of Erk mitogen-activated protein kinase proteins by vascular serotonin receptors publication-title: J. Cardiovasc Pharmacol. contributor: fullname: Baez – volume: 303 start-page: 179 year: 2002 end-page: 187 ident: bib8 article-title: 5-Hydroxytryptamine(2B) receptor function is enhanced in the N(omega)-nitro-L-arginine hypertensive rat publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Watts – volume: 44 start-page: 51 year: 1998 end-page: 63 ident: bib4 article-title: Nitric oxide is produced via 5-HT1B and 5-HT2B receptor activation in human coronary artery endothelial cells publication-title: Kobe J. Med. Sci. contributor: fullname: Yokoyama – volume: 370 start-page: 215 year: 1995 end-page: 221 ident: bib2 article-title: Expression of serotonin receptor mRNAs in blood vessels publication-title: FEBS Lett. contributor: fullname: Lubbert – volume: 312 start-page: 1882 year: 2006 end-page: 1883 ident: bib17 article-title: Cell signaling. H publication-title: Science contributor: fullname: Rhee – volume: 3 start-page: 12 year: 2003 ident: bib7 article-title: Arterial expression of 5-HT2B and 5-HT1B receptors during development of DOCA-salt hypertension publication-title: BMC Pharmacol. contributor: fullname: Watts – volume: 25 start-page: 832 year: 1998 end-page: 838 ident: bib20 article-title: An SOD-mimicry mechanism underlies the role of nitroxides in protecting papain from oxidative inactivation publication-title: Free Radic. Biol. Med. contributor: fullname: Samuni – volume: 81 start-page: 1056 year: 2003 end-page: 1063 ident: bib15 article-title: Attenuation of the serotonin-induced increase in intracellular calcium in rat aortic smooth muscle cells by sarpogrelate publication-title: Can. J. Physiol. Pharmacol. contributor: fullname: Dhalla – volume: 304 start-page: 386 year: 1993 end-page: 391 ident: bib23 article-title: Vanadate-induced contraction of smooth muscle and enhanced protein tyrosine phosphorylation publication-title: Arch. Biochem. Biophys. contributor: fullname: Lauer – volume: 586 start-page: 3855 year: 2008 end-page: 3869 ident: bib10 article-title: c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta publication-title: J. Physiol. contributor: fullname: Toro – volume: 2012 start-page: 398406 year: 2012 ident: bib9 article-title: 5HT(2A) and 5HT(2B) receptors contribute to serotonin-induced vascular dysfunction in diabetes publication-title: Exp. Diabetes Res. contributor: fullname: Lamping – volume: 176 start-page: 47 year: 1997 end-page: 51 ident: bib24 article-title: Smooth muscle contractility and protein tyrosine phosphorylation publication-title: Mol. Cell Biochem. contributor: fullname: St-Louis – volume: 45 start-page: 1340 year: 2008 end-page: 1351 ident: bib26 article-title: Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production publication-title: Free Radic. Biol. Med. contributor: fullname: Griendling – volume: 64 start-page: 359 year: 2012 ident: 10.1016/j.bbrc.2016.12.079_bib3 article-title: Serotonin and blood pressure regulation publication-title: Pharmacol. Rev. doi: 10.1124/pr.111.004697 contributor: fullname: Watts – volume: 61 start-page: 65 year: 2009 ident: 10.1016/j.bbrc.2016.12.079_bib14 article-title: Development of an optimized protocol for primary culture of smooth muscle cells from rat thoracic aortas publication-title: Cytotechnology doi: 10.1007/s10616-009-9236-6 contributor: fullname: Xu – volume: 81 start-page: 1056 year: 2003 ident: 10.1016/j.bbrc.2016.12.079_bib15 article-title: Attenuation of the serotonin-induced increase in intracellular calcium in rat aortic smooth muscle cells by sarpogrelate publication-title: Can. J. Physiol. Pharmacol. doi: 10.1139/y03-108 contributor: fullname: Saini – volume: 303 start-page: 179 year: 2002 ident: 10.1016/j.bbrc.2016.12.079_bib8 article-title: 5-Hydroxytryptamine(2B) receptor function is enhanced in the N(omega)-nitro-L-arginine hypertensive rat publication-title: J. Pharmacol. Exp. Ther. doi: 10.1124/jpet.102.037390 contributor: fullname: Russell – volume: 594 start-page: 57 year: 2010 ident: 10.1016/j.bbrc.2016.12.079_bib19 article-title: Identification of ROS using oxidized DCFDA and flow-cytometry publication-title: Methods Mol. Biol. doi: 10.1007/978-1-60761-411-1_4 contributor: fullname: Eruslanov – volume: 39 start-page: 394 year: 2002 ident: 10.1016/j.bbrc.2016.12.079_bib29 article-title: Upregulation of arterial serotonin 1B and 2B receptors in deoxycorticosterone acetate-salt hypertension publication-title: Hypertension doi: 10.1161/hy02t2.102793 contributor: fullname: Banes – volume: 46 start-page: 600 year: 2013 ident: 10.1016/j.bbrc.2016.12.079_bib27 article-title: Feasibility of simultaneous measurement of cytosolic calcium and hydrogen peroxide in vascular smooth muscle cells publication-title: BMB Rep. doi: 10.5483/BMBRep.2013.46.12.103 contributor: fullname: Chang – volume: 59 start-page: 75 year: 2007 ident: 10.1016/j.bbrc.2016.12.079_bib18 article-title: RNAi therapeutics: principles, prospects and challenges publication-title: Adv. Drug Deliv. Rev. doi: 10.1016/j.addr.2007.03.005 contributor: fullname: Aagaard – volume: 44 start-page: 51 year: 1998 ident: 10.1016/j.bbrc.2016.12.079_bib4 article-title: Nitric oxide is produced via 5-HT1B and 5-HT2B receptor activation in human coronary artery endothelial cells publication-title: Kobe J. Med. Sci. contributor: fullname: Ishida – volume: 25 start-page: 832 year: 1998 ident: 10.1016/j.bbrc.2016.12.079_bib20 article-title: An SOD-mimicry mechanism underlies the role of nitroxides in protecting papain from oxidative inactivation publication-title: Free Radic. Biol. Med. doi: 10.1016/S0891-5849(98)00162-2 contributor: fullname: Offer – volume: 370 start-page: 215 year: 1995 ident: 10.1016/j.bbrc.2016.12.079_bib2 article-title: Expression of serotonin receptor mRNAs in blood vessels publication-title: FEBS Lett. doi: 10.1016/0014-5793(95)00828-W contributor: fullname: Ullmer – volume: 3 start-page: 12 year: 2003 ident: 10.1016/j.bbrc.2016.12.079_bib7 article-title: Arterial expression of 5-HT2B and 5-HT1B receptors during development of DOCA-salt hypertension publication-title: BMC Pharmacol. doi: 10.1186/1471-2210-3-12 contributor: fullname: Banes – volume: 45 start-page: 1340 year: 2008 ident: 10.1016/j.bbrc.2016.12.079_bib26 article-title: Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2008.08.013 contributor: fullname: Dikalov – volume: 38 start-page: 539 year: 2001 ident: 10.1016/j.bbrc.2016.12.079_bib1 article-title: Activation of Erk mitogen-activated protein kinase proteins by vascular serotonin receptors publication-title: J. Cardiovasc Pharmacol. doi: 10.1097/00005344-200110000-00006 contributor: fullname: Watts – volume: 33 start-page: 297 year: 2011 ident: 10.1016/j.bbrc.2016.12.079_bib16 article-title: ROS formation and antioxidant status in brain areas of rats exposed to sodium metavanadate publication-title: Neurotoxicol. Teratol. doi: 10.1016/j.ntt.2010.10.010 contributor: fullname: Cuesta – volume: 56 start-page: 216 year: 2012 ident: 10.1016/j.bbrc.2016.12.079_bib22 article-title: Targeting NADPH oxidases in vascular pharmacology publication-title: Vasc. Pharmacol. doi: 10.1016/j.vph.2012.02.012 contributor: fullname: Schramm – volume: 309 start-page: 711 year: 2004 ident: 10.1016/j.bbrc.2016.12.079_bib12 article-title: Oxidation of protein tyrosine phosphatases as a pharmaceutical mechanism of action: a study using 4-hydroxy-3,3-dimethyl-2H-benzo[g]indole-2,5(3H)-dione publication-title: J. Pharmacol. Exp. Ther. doi: 10.1124/jpet.103.062745 contributor: fullname: Liljebris – volume: 304 start-page: 386 year: 1993 ident: 10.1016/j.bbrc.2016.12.079_bib23 article-title: Vanadate-induced contraction of smooth muscle and enhanced protein tyrosine phosphorylation publication-title: Arch. Biochem. Biophys. doi: 10.1006/abbi.1993.1366 contributor: fullname: Di Salvo – volume: 312 start-page: 1882 year: 2006 ident: 10.1016/j.bbrc.2016.12.079_bib17 article-title: Cell signaling. H2O2, a necessary evil for cell signaling publication-title: Science doi: 10.1126/science.1130481 contributor: fullname: Rhee – volume: 277 start-page: 1103 year: 1996 ident: 10.1016/j.bbrc.2016.12.079_bib28 article-title: The 5-hydroxytryptamine2B receptor and 5-HT receptor signal transduction in mesenteric arteries from deoxycorticosterone acetate-salt hypertensive rats publication-title: J. Pharmacol. Exp. Ther. contributor: fullname: Watts – volume: 176 start-page: 47 year: 1997 ident: 10.1016/j.bbrc.2016.12.079_bib24 article-title: Smooth muscle contractility and protein tyrosine phosphorylation publication-title: Mol. Cell Biochem. doi: 10.1023/A:1006818810678 contributor: fullname: Srivastava – volume: 19 start-page: 441 year: 2001 ident: 10.1016/j.bbrc.2016.12.079_bib25 article-title: Role of c-Src in the regulation of vascular contraction and Ca2+ signaling by angiotensin II in human vascular smooth muscle cells publication-title: J. Hypertens. doi: 10.1097/00004872-200103000-00012 contributor: fullname: Touyz – volume: 26 start-page: 593 year: 2004 ident: 10.1016/j.bbrc.2016.12.079_bib11 article-title: Oxidative stress in hypertension publication-title: Clin. Exp. Hypertens. doi: 10.1081/CEH-200031904 contributor: fullname: de Champlain – volume: 2012 start-page: 398406 year: 2012 ident: 10.1016/j.bbrc.2016.12.079_bib9 article-title: 5HT(2A) and 5HT(2B) receptors contribute to serotonin-induced vascular dysfunction in diabetes publication-title: Exp. Diabetes Res. doi: 10.1155/2012/398406 contributor: fullname: Nelson – volume: 586 start-page: 3855 year: 2008 ident: 10.1016/j.bbrc.2016.12.079_bib10 article-title: c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta publication-title: J. Physiol. doi: 10.1113/jphysiol.2008.153593 contributor: fullname: Lu – volume: 309 start-page: 165 year: 2004 ident: 10.1016/j.bbrc.2016.12.079_bib6 article-title: Characterization of the contractile 5-hydroxytryptamine receptor in the renal artery of the normotensive rat publication-title: J. Pharmacol. Exp. Ther. doi: 10.1124/jpet.103.062562 contributor: fullname: Watts – volume: 69 start-page: 3159 year: 2012 ident: 10.1016/j.bbrc.2016.12.079_bib21 article-title: VAS2870 is a pan-NADPH oxidase inhibitor publication-title: Cell Mol. Life Sci. doi: 10.1007/s00018-012-1107-1 contributor: fullname: Wingler – volume: 128 start-page: 13 year: 1999 ident: 10.1016/j.bbrc.2016.12.079_bib5 article-title: Functional characterization of agonists at recombinant human 5-HT2A, 5-HT2B and 5-HT2C receptors in CHO-K1 cells publication-title: Br. J. Pharmacol. doi: 10.1038/sj.bjp.0702751 contributor: fullname: Porter – volume: 272 start-page: 843 year: 1997 ident: 10.1016/j.bbrc.2016.12.079_bib13 article-title: Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.2.843 contributor: fullname: Huyer |
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SubjectTerms | 5-HT2B receptors Animals Aorta - drug effects Aorta - enzymology Aorta - physiology Calcium Calcium Signaling - drug effects Cell Separation Hypertension Indoles - pharmacology Male Mesenteric Arteries - drug effects Mesenteric Arteries - physiology Muscle Contraction - drug effects Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - enzymology Oxidative Stress Protein tyrosine phosphatases Protein Tyrosine Phosphatases - analysis Rats Rats, Wistar Receptor, Serotonin, 5-HT2B - genetics Receptor, Serotonin, 5-HT2B - metabolism RNA, Messenger - analysis Serotonin 5-HT2 Receptor Agonists - pharmacology Smooth muscle cells Thiophenes - pharmacology Vanadates - pharmacology Vasoconstriction - drug effects Vasoconstriction - physiology Vasoconstrictor Agents - pharmacology |
Title | Inhibition of protein tyrosine phosphatases unmasks vasoconstriction and potentiates calcium signaling in rat aorta smooth muscle cells in response to an agonist of 5-HT2B receptors BW723C86 |
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