An Iron-Sulfur Cluster in the C-terminal Domain of the p58 Subunit of Human DNA Primase

• Published in: The Journal of biological chemistry Vol. 282; no. 46; pp. 33444 - 33451
• Main Authors: Weiner, Brian E., Huang, Hao, Dattilo, Brian M., Nilges, Mark J., Fanning, Ellen, Chazin, Walter J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.11.2007; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Circular Dichroism; Cysteine - chemistry; DNA - chemistry; DNA Primase - chemistry; DNA Primase - metabolism; DNA Primase - physiology; Electron Spin Resonance Spectroscopy; Humans; Iron-Sulfur Proteins - chemistry; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Protein Structure, Tertiary; Recombinant Proteins - chemistry; Sequence Homology, Amino Acid; Spectrophotometry - methods; Spectrophotometry, Ultraviolet

DNA primase synthesizes short RNA primers that are required to initiate DNA synthesis on the parental template strands during DNA replication. Eukaryotic primase contains two subunits, p48 and p58, and is normally tightly associated with DNA polymerase α. Despite the fundamental importance of primas...