The existence of aspolin and its trimethylamine‐N‐oxide demethylating activity in the muscle of freshwater fish

• Published in: Fisheries science Vol. 71; no. 4; pp. 904 - 913
• Main Authors: KIMURA, Meiko, TAKEUCHI, Kazuharu, KIMURA, Ikuo, SEKI, Nobuo
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Science Pty 01.08.2005; Springer Nature B.V
• Subjects: Amino acids; aspolin; Carp; Cyprinus carpio; Electrophoresis; Enzymatic activity; Freshwater; Freshwater fish; polyaspartic acid; Polymers; Proteins; Residues; Theragra chalcogramma; trimethylamine‐N‐oxide; trimethylamine‐N‐oxide demethylase; walleye pollack

Aspolin is a polyaspartic acid‐like protein, which is originally isolated from walleye pollack Theragra chalcogramma muscle as trimethylamine‐N‐oxide (TMAO) demethylase. Although carp Cyprinus carpio muscle contains a trace amount of the enzyme substrate, TMAO, aspolin can be extracted and purified...