A convenient assay for estimating the possible involvement of efflux of fluoroquinolones by Streptococcus pneumoniae and Staphylococcus aureus : Evidence for diminished moxifloxacin, sparfloxacin, and trovafloxacin efflux

• Published in: Antimicrobial agents and chemotherapy Vol. 44; no. 3; pp. 798 - 801
• Main Authors: BEYER, R, PESTOVA, E, MILLICHAP, J. J, STOSOR, V, NOSKIN, G. A, PETERSON, L. R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2000
• Series: Note
• Subjects: Anti-Infective Agents - metabolism; Anti-Infective Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Aza Compounds; Biological and medical sciences; Drug Resistance, Microbial; Fluoroquinolones; Mechanisms of Resistance; Medical sciences; Microbial Sensitivity Tests; Moxifloxacin; Naphthyridines - metabolism; Naphthyridines - pharmacology; Pharmacology. Drug treatments; Quinolines; Reserpine; Reserpine - pharmacology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - metabolism; Streptococcus pneumoniae; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - metabolism; Microorganism growth; Trovafloxacin; Moxifloxacin; In vitro; Biological activity; Streptococcus pneumoniae; Resistance; Streptococcaceae; Alkaloid; Fluoroquinolone derivatives; Bacteria; Micrococcales; Micrococcaceae; Mutation; Sparfloxacin; Antibacterial agent; Quinolone derivatives; Reserpine; Staphylococcus aureus
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/AAC.44.3.798-801.2000

We developed a simplified assay for estimating efflux by measuring the effect of reserpine on the growth of Streptococcus pneumoniae and Staphylococcus aureus over 7 h. Reserpine enhanced ciprofloxacin and levofloxacin 17 to 68%. The hydrophobic drug trovafloxacin and the drug moxifloxacin, with a bulky C-7 substituent but hydrophilicity similar to that of levofloxacin, showed little (0 to 11%) reserpine-enhancing effect. The ease of resistant mutant strain selection correlated with efflux susceptibility.