Iron incorporation into apoferritin. The role of apoferritin as a ferroxidase

• Published in: The Journal of biological chemistry Vol. 261; no. 28; pp. 13182 - 13185
• Main Authors: Bakker, G R, Boyer, R F
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.10.1986; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Apoferritins - metabolism; Binding Sites; Biological and medical sciences; Cell physiology; Ferric Compounds - metabolism; Ferritins - analogs & derivatives; Ferrous Compounds - metabolism; Fundamental and applied biological sciences. Psychology; Horses; Humans; Iron - metabolism; Kinetics; Membrane and intracellular transports; Molecular and cellular biology; Oxidoreductases - metabolism; Quaternary Ammonium Compounds - metabolism; Transferrin - metabolism; Incorporation; Enzymatic activity; Ferritin; Oxidase; Coeruloplasmin; Ion transport; Molecular interaction; Iron; Mechanism

Apoferritin catalyzes the oxidation of Fe(II) to Fe(III). Ferroxidase activity is assayed and characterized by coupling the oxidation with the binding of Fe(III) to transferrin. The initial rate of Fe(II) oxidation is dependent on apoferritin and initial Fe(II) concentration but independent of trans...