Evaluation of porcine GM-CSF during PRRSV infection in vitro and in vivo indicating a protective role of GM-CSF related with M1 biased activation in alveolar macrophage during PRRSV infection

• Published in: Frontiers in immunology Vol. 13; p. 967338
• Main Authors: Ji, Qi, Qu, Guanggang, Liu, Bing, Bai, Yang, Wang, Guihua, Chen, Rui, Zheng, Xu, Zhang, Zhigang, Yang, Yonglin, Wu, Chunyan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 19.10.2022
• Subjects: Animals; ELISA; Gene Expression Profiling; GM-CSF; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism; immune response; Immunology; macrophage activation; Macrophages, Alveolar; Porcine respiratory and reproductive syndrome virus - genetics; PRRSV; RNA, Messenger - metabolism; Swine; macrophage activation; GM-CSF; immune response; PRRSV; ELISA

Granulocyte-macrophage colony stimulating factor (GM-CSF), participates in diverse biological processes associated with innate and adaptive immunity, has unknown effects during PRRSV infection. Here, a double-antibody sandwich ELISA for pGM-CSF was developed in-house for evaluation of pGM-CSF level...