Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks

Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus t...

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Published inFrontiers in molecular biosciences Vol. 7; p. 582499
Main Authors Srivastava, Sonal, Upadhyay, Dilip J, Srivastava, Ashish
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.12.2020
Subjects
Cas12
Cas13
Cas9
CRISPR associated proteins
isothermal amplification
Molecular Biosciences
NAT
NAT
Cas13
CRISPR associated proteins
Cas12
Cas9
isothermal amplification
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Abstract Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus tristeza virus, and Tomato yellow leaf curl virus have paralyzed the economy of many countries. The cure for major viral diseases is not feasible; however, early detection and surveillance of the disease can obstruct their spread. Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Several rapid nucleic acid diagnostic kits have been developed and validated using Cas9, Cas12, and Cas13 proteins. This review summarizes the CRISPR/Cas-based next-generation molecular diagnostic techniques and portability of devices for field-based utilization.
AbstractList Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus tristeza virus, and Tomato yellow leaf curl virus have paralyzed the economy of many countries. The cure for major viral diseases is not feasible; however, early detection and surveillance of the disease can obstruct their spread. Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Several rapid nucleic acid diagnostic kits have been developed and validated using Cas9, Cas12, and Cas13 proteins. This review summarizes the CRISPR/Cas-based next-generation molecular diagnostic techniques and portability of devices for field-based utilization.
Author Srivastava, Ashish
Srivastava, Sonal
Upadhyay, Dilip J
AuthorAffiliation 1 Amity Institute of Virology and Immunology, Amity University Uttar Pradesh , Noida , India
2 Amity University Uttar Pradesh , Noida , India
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Keywords NAT
Cas13
CRISPR associated proteins
Cas12
Cas9
isothermal amplification
Language English
License Copyright © 2020 Srivastava, Upadhyay and Srivastava.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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Reviewed by: Sangeeta Chakraborty, National Jewish Health, United States; Allen Thayakumar Basanthakumar, Dana–Farber Cancer Institute, United States
These authors have contributed equally to this work
Edited by: Megha Agrawal, University of Illinois at Chicago, United States
This article was submitted to Molecular Diagnostics and Therapeutics, a section of the journal Frontiers in Molecular Biosciences
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Snippet Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new...
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SubjectTerms Cas12
Cas13
Cas9
CRISPR associated proteins
isothermal amplification
Molecular Biosciences
NAT
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Title Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks
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