Fabrication of highly interconnected porous silk fibroin scaffolds for potential use as vascular grafts

• Published in: Acta biomaterialia Vol. 10; no. 5; pp. 2014 - 2023
• Main Authors: Zhu, Meifeng, Wang, Kai, Mei, Jingjing, Li, Chen, Zhang, Jiamin, Zheng, Wenting, An, Di, Xiao, Nannan, Zhao, Qiang, Kong, Deling, Wang, Lianyong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2014
• Subjects: Alcohols - pharmacology; Animals; Blood Vessel Prosthesis; Bombyx; Cell Adhesion - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Compressive Strength - drug effects; Fibroins - pharmacology; Freeze-drying; Freezing; Heparin; Heparin - pharmacology; Humans; Materials Testing - methods; Mice; Microscopy, Electron, Scanning; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; Neovascularization; Neovascularization, Physiologic - drug effects; Porosity; Prothrombin Time; Silk fibroin scaffolds; Spectroscopy, Fourier Transform Infrared; Tissue Scaffolds - chemistry; Vascular graft; Neovascularization; Heparin; Freeze-drying; Vascular graft; Silk fibroin scaffolds

In this study, an easy-to-adopt and mild approach based on modified freeze-drying method was developed to fabricate a silk fibroin (SF) scaffold with high porosity, proper pore size and highly interconnected pores. The preparation conditions were optimized by pre-selecting the freezing temperature,...