Broad T Cell Targeting of Structural Proteins After SARS-CoV-2 Infection: High Throughput Assessment of T Cell Reactivity Using an Automated Interferon Gamma Release Assay

• Published in: Frontiers in immunology Vol. 12; p. 688436
• Main Authors: Brand, Isabel, Gilberg, Leonard, Bruger, Jan, Garí, Mercè, Wieser, Andreas, Eser, Tabea M., Frese, Jonathan, Ahmed, Mohamed I. M., Rubio-Acero, Raquel, Guggenbuehl Noller, Jessica M., Castelletti, Noemi, Diekmannshemke, Jana, Thiesbrummel, Sophie, Huynh, Duc, Winter, Simon, Kroidl, Inge, Fuchs, Christiane, Hoelscher, Michael, Roider, Julia, Kobold, Sebastian, Pritsch, Michael, Geldmacher, Christof
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 20.05.2021
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Viral - blood; COVID-19; COVID-19 - blood; COVID-19 - immunology; Female; high through put; Humans; Immunology; interferon gamma release assay (IGRA); Interferon-gamma - immunology; Interferon-gamma Release Tests; Male; Middle Aged; SARS-CoV-2; SARS-CoV-2 - immunology; T cell response; T-Lymphocytes - immunology; Viral Structural Proteins - immunology; Young Adult; COVID-19; SARS-CoV-2; high through put; interferon gamma release assay (IGRA); T cell response

Adaptive immune responses to structural proteins of the virion play a crucial role in protection against coronavirus disease 2019 (COVID-19). We therefore studied T cell responses against multiple SARS-CoV-2 structural proteins in a large cohort using a simple, fast, and high-throughput approach. An...