A Small-Scale shRNA Screen in Primary Mouse Macrophages Identifies a Role for the Rab GTPase Rab1b in Controlling Salmonella Typhi Growth

• Published in: Frontiers in cellular and infection microbiology Vol. 11; p. 660689
• Main Authors: Solano-Collado, Virtu, Colamarino, Rosa Angela, Calderwood, David A, Baldassarre, Massimiliano, Spanò, Stefania
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 07.04.2021
• Subjects: Animals; Cellular and Infection Microbiology; host-defense; macrophages; Macrophages - metabolism; Mice; rab GTP-Binding Proteins - metabolism; Rab GTPases; Rab1b; RNA, Small Interfering; Salmonella Typhi; Salmonella typhi - genetics; shRNA screen; Typhoid Fever; macrophages; shRNA screen; host-defense; Rab GTPases; Rab1b; Salmonella Typhi

Typhi is a human-restricted bacterial pathogen that causes typhoid fever, a life-threatening systemic infection. A fundamental aspect of . Typhi pathogenesis is its ability to survive in human macrophages but not in macrophages from other animals (i.e. mice). Despite the importance of macrophages in...