Identification of sugar transporter genes and their roles in the pathogenicity of Verticillium dahliae on cotton
Verticillium wilt (VW) caused by is a soil-borne vascular fungal disease that severely affects cotton yield and fiber quality. Sugar metabolism plays an important role in the growth and pathogenicity of . However, limited information is known about the sugar transporter genes and their roles in the...
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Published in | Frontiers in plant science Vol. 14; p. 1123523 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
26.01.2023
|
Subjects | |
Online Access | Get full text |
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Summary: | Verticillium wilt (VW) caused by
is a soil-borne vascular fungal disease that severely affects cotton yield and fiber quality. Sugar metabolism plays an important role in the growth and pathogenicity of
. However, limited information is known about the sugar transporter genes and their roles in the growth and pathogenicity of
.
In this study, genome-wide identification of sugar transporter genes in
was conducted and the expression profiles of these genes in response to root exudates from cotton varieties susceptible or resistant to
were investigated based on RNA-seq data. Tobacco Rattle Virus-based host-induced gene silencing (TRV-based HIGS) and artificial small interfering RNAs (asiRNAs) were applied to investigate the function of candidate genes involved in the growth and pathogenic process of
.
A total of 65 putative sugar transporter genes were identified and clustered into 8 Clades. Of the 65 sugar transporter genes, 9 were found to be induced only by root exudates from the susceptible variety, including
and
that were selected for further functional study. Silencing of
or
in host plants by TRV-based HIGS reduced fungal biomass and enhanced cotton resistance against
. Additionally, silencing of
and
by feeding asiRNAs targeting
(asiR815 or asiR1436) and
(asiR201 or asiR1238) inhibited fungal growth, exhibiting significant reduction in hyphae and colony diameter, with a more significant effect observed for the asiRNAs targeting
. The inhibitory effect of asiRNAs on the growth of
was enhanced with the increasing concentration of asiRNAs. Silencing of
by feeding asiR815+asiR1436 significantly decreased the pathogenicity of
.
The results suggest that
and
are sugar transporter genes required for growth and pathogenicity of
and that asiRNA is a valuable tool for functional characterization of
genes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Xiaofeng Su, Institute of Biotechnology (CAAS) Beijing, China; Zhiqiang Li, Institute of Plant Protection (CAAS), China Edited by: Tao Zhang, Institute of Microbiology (CAS), China This article was submitted to Plant Pathogen Interactions, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2023.1123523 |