Inhibitory effect of (-)-epigallocatechin gallate on titanium particle-induced TNF-α release and in vivo osteolysis

• Published in: Experimental & molecular medicine Vol. 43; no. 7; pp. 411 - 418
• Main Authors: Jin, Shan, Park, Ju-Young, Hong, Jung-Min, Kim, Tae-Ho, Shin, Hong-In, Park, Eui Kyun, Kim, Shin-Yoon
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.07.2011; Korean Society for Biochemistry and Molecular Biology; 생화학분자생물학회
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Catechin - analogs & derivatives; Catechin - pharmacology; Cell Line; Implants, Experimental; Macrophages - drug effects; Macrophages - metabolism; Male; Medical Biochemistry; Mice; Mice, Inbred C57BL; Mitogen-Activated Protein Kinase 8 - metabolism; Molecular Medicine; NF-kappa B - metabolism; Original; Osteolysis - chemically induced; Osteolysis - metabolism; Osteolysis - prevention & control; Particulate Matter - adverse effects; Prosthesis Failure; Signal Transduction - drug effects; Skull - drug effects; Skull - pathology; Stem Cells; Titanium - adverse effects; Transcription Factor AP-1 - metabolism; Tumor Necrosis Factor-alpha - metabolism; 생화학; epigallocatechin gallate; transcription factor AP-1; mitogen-activated protein kinases; NF-κB, titanium; tumor necrosis factor-α
• ISSN: 1226-3613; 2092-6413; 2092-6413
• DOI: 10.3858/emm.2011.43.7.045

Tumor necrosis factor-α (TNF-α) and inflammatory cytokines released from activated macrophages in response to particulate debris greatly impact periprosthetic bone loss and consequent implant failure. In the present study, we found that a major polyphenolic component of green tea, (-)-epigallocatechin gallate (EGCG), inhibited Ti particle-induced TNF-α release in macrophages in vitro and calvarial osteolysis in vivo . The Ti stimulation of macrophages released TNF-α in a dose- and time-dependent manner, and EGCG substantially suppressed Ti particle-induced TNF-α release. Analysis of signaling pathway showed that EGCG inhibited the Ti-induced c-Jun N-terminus kinase (JNK) activation and inhibitory κB (IκB) degradation, and consequently the Ti-induced transcriptional activation of AP-1 and NF-κB. In a mouse calvarial osteolysis model, EGCG inhibited Ti particle-induced osteolysis in vivo by suppressing TNF-α expression and osteoclast formation. Therefore, EGCG may be a potential candidate compound for osteolysis prevention and treatment as well as aseptic loosening after total replacement arthroplasty.