Acute Cold Water-Immersion Restraint Stress Induces Intestinal Injury and Reduces the Diversity of Gut Microbiota in Mice

• Published in: Frontiers in cellular and infection microbiology Vol. 11; p. 706849
• Main Authors: Zhang, Yuan, Wu, Shuwen, Liu, Yongming, Ma, Jingchang, Li, Wenpeng, Xu, Xuexue, Wang, Yuling, Luo, Yanling, Cheng, Kun, Zhuang, Ran
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.10.2021
• Subjects: Animals; Cellular and Infection Microbiology; cold water-immersion restraint stress; Cold-Shock Response; Gastrointestinal Microbiome; gut microbiota; Immersion; inflammation; intestinal injury; Intestinal Mucosa; Male; Mice; Mice, Inbred C57BL; mouse models; RNA, Ribosomal, 16S - genetics; Water; cold water-immersion restraint stress; mouse models; inflammation; gut microbiota; intestinal injury
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2021.706849

Growing evidence has demonstrated that stress triggers gastrointestinal (GI) disorders. This study aimed to investigate how the acute cold water-immersion restraint (CWIR) stress affects intestinal injury and gut microbiota (GM) distribution. Male C57BL/6 mice were used to establish a CWIR animal model. Hematoxylin–eosin and periodic acid–Schiff staining were performed to assess intestinal histopathological changes. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis and immunofluorescence staining were used to evaluate the expression of inflammatory cytokines and immune cell infiltration in the intestinal tissues. The gut permeability and intestinal occludin protein expression were determined through fluorescein isothiocyanate-dextran detection and western blot, respectively. GM profiles were analyzed via high-throughput sequencing of the fecal bacterial 16S rRNA genes. Results showed that CWIR induced more severe intestinal mucosal injury compared to the control, leading to a significant increase in tumor necrosis factor-α expression, but no infiltration of neutrophil and T cells. CWIR also resulted in GI disruption and increased the permeability of the intestinal mucosa. GM profiles showed that CWIR reduced GM diversity of mice compared with the control group. Specifically, aerobic and gram-negative bacteria significantly increased after CWIR, which was associated with the severity of gut injury under stress. Therefore, acute CWIR leads to severe intestinal damage with inflammation and disrupts the GM homeostasis, contributing to decreased GM diversity. Our findings provide the theoretical basis for the further treatment of intestinal disorders induced by CWIR.