Commercial scale genetic transformation of mature seed embryo explants in maize

• Published in: Frontiers in plant science Vol. 13; p. 1056190
• Main Authors: Ye, Xudong, Shrawat, Ashok, Williams, Edward, Rivlin, Anatoly, Vaghchhipawala, Zarir, Moeller, Lorena, Kumpf, Jennifer, Subbarao, Shubha, Martinell, Brian, Armstrong, Charles, Saltarikos, M Annie, Somers, David, Chen, Yurong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 29.11.2022
• Subjects: Agrobacterium; maize transformation; meristem; organogenesis; Plant Science; seed embryo explants (SEEs); Zea mays; Agrobacterium; Zea mays; meristem; genotype-flexible; maize transformation; seed embryo explants (SEEs); organogenesis

A novel, efficient maize genetic transformation system was developed using -mediated transformation of embryo explants from mature seeds. Seeds from field grown plants were sterilized and crushed to isolate embryo explants consisting of the coleoptile, leaf primordia, and shoot apical meristem which...