Micro scale self-interaction chromatography of proteins: A mAb case-study

• Published in: Journal of Chromatography A Vol. 1434; pp. 57 - 63
• Main Authors: Hedberg, S.H.M., Heng, J.Y.Y., Williams, D.R., Liddell, J.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.02.2016
• Subjects: Animals; Antibodies, Monoclonal - chemistry; Automation; Chickens; Chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Formulation; Liquid chromatography; Monoclonal antibodies; Muramidase - chemistry; Protein stability; Proteins; Scale-down; Screening; Second virial coefficient; Self-interaction chromatography; Monoclonal antibodies; Second virial coefficient; Scale-down; Protein stability; Self-interaction chromatography; Formulation

•Describes a methodology for micro scale SIC using 10 times less protein.•Examine a number columns of different sizes/aspect ratios.•Calculated B22 using a variety of conditions for all the columns for mAbs.•Similar results were obtained for all the different columns.•Guidance given on amount of pro...