Allosteric Inhibition of a Zinc-Sensing Transcriptional Repressor: Insights into the Arsenic Repressor (ArsR) Family

• Published in: Journal of molecular biology Vol. 425; no. 7; pp. 1143 - 1157
• Main Authors: Campanello, Gregory C., Ma, Zhen, Grossoehme, Nicholas E., Guerra, Alfredo J., Ward, Brian P., DiMarchi, Richard D., Ye, Yuzhen, Dann, Charles E., Giedroc, David P.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 12.04.2013
• Subjects: Allosteric Regulation; allostery; Amino Acid Substitution; ArsR; Bacterial Proteins; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites; Binding Sites - genetics; Binding, Competitive; chemistry; Crystallography, X-Ray; DNA; DNA-Binding Proteins; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; energy; evolution; genetics; Hydrogen Bonding; Kinetics; metabolism; metal homeostasis; metal ions; Models, Molecular; mutants; Mutation; Protein Binding; Protein Multimerization; Protein Structure, Secondary; Protein Structure, Tertiary; proteins; Repressor Proteins; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus aureus - metabolism; statistical coupling analysis; Thermodynamics; transcription (genetics); Transcription, Genetic; zinc; Zinc - chemistry; Zinc - metabolism; zinc sensor; SCA; TROSY; statistical coupling analysis; zinc sensor; ITC; metal homeostasis; HSQC; TCEP; allostery; ArsR

The molecular basis of allosteric regulation remains a subject of intense interest. Staphylococcus aureus CzrA is a member of the ubiquitous arsenic repressor (ArsR) family of bacterial homodimeric metal-sensing proteins and has emerged as a model system for understanding allosteric regulation of op...