Reproducible High Level Infection of Cultured Adult Human Hepatocytes by Hepatitis B Virus: Effect of Polyethylene Glycol on Adsorption and Penetration

• Published in: Virology (New York, N.Y.) Vol. 192; no. 2; pp. 534 - 540
• Main Authors: Gripon, Philippe, Diot, Christian, Guguen-Guillouzo, Christiane
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.02.1993; Elsevier
• Subjects: Adult; Biological and medical sciences; Blotting, Southern; Cells, Cultured; DNA, Viral - genetics; DNA, Viral - isolation & purification; Fundamental and applied biological sciences. Psychology; Hepatitis B e Antigens - analysis; Hepatitis B e Antigens - biosynthesis; Hepatitis B Surface Antigens - analysis; Hepatitis B Surface Antigens - biosynthesis; hepatitis B virus; Hepatitis B virus - drug effects; Hepatitis B virus - physiology; Humans; Kinetics; Liver - cytology; Liver - microbiology; Microbiology; Polyethylene Glycols - pharmacology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Viral Proteins - analysis; Viral Proteins - biosynthesis; Virology; Virus Replication; Virus; Ethylene oxide polymer; Human; Infection; Cell culture; Hepatocyte; Hepadnaviridae; Virus replication cycle; Hepatitis B virus; In vitro; Host virus relation; Virus penetration

We have previously succeeded in infecting normal human hepatocyte primary cultures with hepatitis B virus (HBV). However, infection was subject to individual variations even in the presence of dimethyl sulfoxide (DMSO), which appeared to increase the amounts of viral DNA associated with the cells. I...