Design of a single protein that spans the entire 2-V range of physiological redox potentials

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 113; no. 2; pp. 262 - 267
• Main Authors: Hosseinzadeh, Parisa, Marshall, Nicholas M., Chacón, Kelly N., Yu, Yang, Nilges, Mark J., New, Siu Yee, Tashkov, Stoyan A., Blackburn, Ninian J., Lu, Yi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 12.01.2016; National Acad Sciences
• Series: From the Cover
• Subjects: Azurin - chemistry; Azurin - metabolism; Bacterial proteins; Biochemistry; Biophysics; Biotechnology; Electrochemical Techniques; Electron Spin Resonance Spectroscopy; Gram-positive bacteria; Models, Molecular; Mutation - genetics; Oxidation-Reduction; Physical Sciences; Physiology; Protein Engineering; Spectrometry, X-Ray Emission; Spectrophotometry, Ultraviolet; Spectrum analysis; secondary coordination sphere; cupredoxins; electron transfer; reduction potential; azurin

The reduction potential (Eo′) is a critical parameter in determining the efficiency of most biological and chemical reactions. Biology employs three classes of metalloproteins to cover the majority of the 2-V range of physiological Eo′s. An ultimate test of our understanding of Eo′ is to find out th...