Repurposing of Anthocyanin Biosynthesis for Plant Transformation and Genome Editing
CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the...
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| Published in | Frontiers in genome editing Vol. 2; p. 607982 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
03.12.2020
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| Abstract | CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology enables us to identify transgenic events even at calli stage, to select transformants with elevated
expression, and to identify transgene-free plants in the field. We used the AAC technology to edit
and
and successfully generated many transgene-free and target gene-edited plants at T1 generation. The AAC technology greatly reduced the labor, time, and costs needed for editing target genes in rice. |
|---|---|
| AbstractList | CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology enables us to identify transgenic events even at calli stage, to select transformants with elevated
expression, and to identify transgene-free plants in the field. We used the AAC technology to edit
and
and successfully generated many transgene-free and target gene-edited plants at T1 generation. The AAC technology greatly reduced the labor, time, and costs needed for editing target genes in rice. CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology enables us to identify transgenic events even at calli stage, to select transformants with elevated Cas9 expression, and to identify transgene-free plants in the field. We used the AAC technology to edit LAZY1 and G1 and successfully generated many transgene-free and target gene-edited plants at T1 generation. The AAC technology greatly reduced the labor, time, and costs needed for editing target genes in rice. CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology enables us to identify transgenic events even at calli stage, to select transformants with elevated Cas9 expression, and to identify transgene-free plants in the field. We used the AAC technology to edit LAZY1 and G1 and successfully generated many transgene-free and target gene-edited plants at T1 generation. The AAC technology greatly reduced the labor, time, and costs needed for editing target genes in rice. |
| Author | Xu, Meilian Wu, Junhua Zhan, Huadong Zhu, Min Zhao, Yunde Wang, Rongchen Ouyang, Lejun Sun, Hui Wang, Lihao He, Yubing Yan, Lang |
| AuthorAffiliation | 5 Section of Cell and Developmental Biology, University of California , San Diego, La Jolla, CA , United States 2 National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University , Wuhan , China 4 Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou , China 3 Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Guangdong University of Petrochemical Technology , Maoming , China 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University , Nanjing , China |
| AuthorAffiliation_xml | – name: 2 National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University , Wuhan , China – name: 3 Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Guangdong University of Petrochemical Technology , Maoming , China – name: 4 Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou , China – name: 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University , Nanjing , China – name: 5 Section of Cell and Developmental Biology, University of California , San Diego, La Jolla, CA , United States |
| Author_xml | – sequence: 1 givenname: Yubing surname: He fullname: He, Yubing organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 2 givenname: Min surname: Zhu fullname: Zhu, Min organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 3 givenname: Junhua surname: Wu fullname: Wu, Junhua organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 4 givenname: Lejun surname: Ouyang fullname: Ouyang, Lejun organization: Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Guangdong University of Petrochemical Technology, Maoming, China – sequence: 5 givenname: Rongchen surname: Wang fullname: Wang, Rongchen organization: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China – sequence: 6 givenname: Hui surname: Sun fullname: Sun, Hui organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 7 givenname: Lang surname: Yan fullname: Yan, Lang organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 8 givenname: Lihao surname: Wang fullname: Wang, Lihao organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 9 givenname: Meilian surname: Xu fullname: Xu, Meilian organization: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China – sequence: 10 givenname: Huadong surname: Zhan fullname: Zhan, Huadong organization: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China – sequence: 11 givenname: Yunde surname: Zhao fullname: Zhao, Yunde organization: Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, United States |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34713232$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao. Copyright © 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao. 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao |
| Copyright_xml | – notice: Copyright © 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao. – notice: Copyright © 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao. 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao |
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| Keywords | CRISPR AAC anthocyanin rice transgene-free |
| Language | English |
| License | Copyright © 2020 He, Zhu, Wu, Ouyang, Wang, Sun, Yan, Wang, Xu, Zhan and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Yiping Qi, University of Maryland, United States; Kan Wang, Iowa State University, United States These authors have contributed equally to this work This article was submitted to Genome Editing in Plants, a section of the journal Frontiers in Genome Editing Edited by: Lanqin Xia, Chinese Academy of Agricultural Sciences, China |
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| Title | Repurposing of Anthocyanin Biosynthesis for Plant Transformation and Genome Editing |
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