Cyclin E Uses Cdc6 as a Chromatin-Associated Receptor Required for DNA Replication

• Published in: The Journal of cell biology Vol. 152; no. 6; pp. 1267 - 1278
• Main Authors: Furstenthal, Laura, Kaiser, Brett K., Swanson, Craig, Jackson, Peter K.
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 19.03.2001; The Rockefeller University Press
• Subjects: Amino Acid Motifs; Animals; Blotting, Western; CDC2-CDC28 Kinases; Cdc6 protein; Cdk2 protein; Cell cycle; Cell Cycle - physiology; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cells; Chromatin; Chromatin - metabolism; Cyclin E - metabolism; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinases - metabolism; Cyclins; Deoxyribonucleic acid; DNA; DNA replication; DNA Replication - drug effects; DNA Replication - physiology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - isolation & purification; DNA-Binding Proteins - metabolism; Enzymes; Humans; Interphase; Male; Mitosis; Models, Biological; Molecular biology; Oocytes - chemistry; Oocytes - physiology; Origin Recognition Complex; Original; Phosphatases; Phosphorylation; Protein Binding; Protein Serine-Threonine Kinases - metabolism; Protein Tyrosine Phosphatases; Proteins; Recombinant Fusion Proteins - metabolism; Saccharomyces cerevisiae Proteins; Spermatozoa; Spermatozoa - cytology; Spermatozoa - physiology; Xenopus laevis; Xenopus Proteins

Using an in vitro chromatin assembly assay in Xenopus egg extract, we show that cyclin E binds specifically and saturably to chromatin in three phases. In the first phase, the origin recognition complex and Cdc6 prereplication proteins, but not the minichromosome maintenance complex, are necessary a...