A novel ATP-generating machinery to counter nitrosative stress is mediated by substrate-level phosphorylation

• Published in: Biochimica et biophysica acta Vol. 1850; no. 1; pp. 43 - 50
• Main Authors: Auger, Christopher, Appanna, Vasu D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2015
• Subjects: Adaptation, Physiological; adenosine triphosphate; Adenosine Triphosphate - metabolism; adenylate kinase; Adenylate Kinase - metabolism; anti-infective agents; bacteria; Bacterial Proteins - metabolism; Chromatography, High Pressure Liquid; citrates; Citric Acid Cycle; Electrophoresis, Polyacrylamide Gel; energy; high performance liquid chromatography; macrophages; Metabolic Networks and Pathways; Metabolism; Metabolon; Microbiology; Models, Biological; molecular conformation; Multienzyme Complexes - metabolism; nitric oxide; Nitric Oxide - metabolism; nitrogen; nucleoside-diphosphate kinase; Nucleoside-Diphosphate Kinase - metabolism; Oxidative Phosphorylation; Oxo-Acid-Lyases - metabolism; pathogens; phosphoenolpyruvate carboxylase; Phosphoenolpyruvate Carboxylase - metabolism; Phosphotransfer; polyacrylamide gel electrophoresis; Pseudomonas; Pseudomonas fluorescens - metabolism; pyruvate phosphate dikinase; Pyruvate, Orthophosphate Dikinase - metabolism; Reactive nitrogen species; Reactive Nitrogen Species - metabolism; Stress, Physiological; tricarboxylic acid cycle; NO; CFE; Microbiology; Metabolon; Reactive nitrogen species; ADP; PEPC; SNP; NDPK; RNS; SLP; Phosphotransfer; HPLC; PPDK; AMP; BN-PAGE; G6PDH; ACK; CL; AK; Metabolism; TCA; ETC; PEP; PK; ATP; DEANO

It is well-known that elevated amounts of nitric oxide and other reactive nitrogen species (RNS) impact negatively on the tricarboxylic acid (TCA) cycle and oxidative phosphorylation. These perturbations severely compromise O2-dependent energy production. While bacteria are known to adapt to RNS, a...