Longitudinal Analysis of T and B Cell Receptor Repertoire Transcripts Reveal Dynamic Immune Response in COVID-19 Patients

• Published in: Frontiers in immunology Vol. 11; p. 582010
• Main Authors: Niu, Xuefeng, Li, Song, Li, Pingchao, Pan, Wenjing, Wang, Qian, Feng, Ying, Mo, Xiaoneng, Yan, Qihong, Ye, Xianmiao, Luo, Jia, Qu, Linbing, Weber, Daniel, Byrne-Steele, Miranda L., Wang, Zhe, Yu, Fengjia, Li, Fang, Myers, Richard M., Lotze, Michael T., Zhong, Nanshan, Han, Jian, Chen, Ling
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 30.09.2020
• Subjects: Adult; Aged; Aged, 80 and over; B cell receptor; B-Lymphocytes - immunology; Betacoronavirus - immunology; biomarker; CD4 Lymphocyte Count; Coronavirus Infections - immunology; Coronavirus Infections - pathology; COVID-19; Female; High-Throughput Nucleotide Sequencing; Humans; immune repertoire; Immunology; Lymphopenia - pathology; Male; Middle Aged; Pandemics; Pneumonia, Viral - immunology; Pneumonia, Viral - pathology; Receptors, Antigen, B-Cell - genetics; Receptors, Antigen, T-Cell - genetics; SARS-CoV-2; T cell receptor; T-Lymphocytes - immunology; COVID-19; SARS-CoV-2; immune repertoire; T cell receptor; B cell receptor; biomarker

Severe COVID-19 is associated with profound lymphopenia and an elevated neutrophil to lymphocyte ratio. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. Surprisingly, TCR repertoires we...