Longitudinal Analysis of T and B Cell Receptor Repertoire Transcripts Reveal Dynamic Immune Response in COVID-19 Patients

Severe COVID-19 is associated with profound lymphopenia and an elevated neutrophil to lymphocyte ratio. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. Surprisingly, TCR repertoires we...

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Published inFrontiers in immunology Vol. 11; p. 582010
Main Authors Niu, Xuefeng, Li, Song, Li, Pingchao, Pan, Wenjing, Wang, Qian, Feng, Ying, Mo, Xiaoneng, Yan, Qihong, Ye, Xianmiao, Luo, Jia, Qu, Linbing, Weber, Daniel, Byrne-Steele, Miranda L., Wang, Zhe, Yu, Fengjia, Li, Fang, Myers, Richard M., Lotze, Michael T., Zhong, Nanshan, Han, Jian, Chen, Ling
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 30.09.2020
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Summary:Severe COVID-19 is associated with profound lymphopenia and an elevated neutrophil to lymphocyte ratio. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. Surprisingly, TCR repertoires were markedly diminished during the early onset of severe disease but recovered during the convalescent stage. Monitoring TCR repertoires could serve as an indicative biomarker to predict disease progression and recovery. Panoramic concurrent assessment of BCR repertoires demonstrated isotype switching and a transient but dramatic early IgA expansion. Dominant B cell clonal expansion with decreased diversity occurred following recovery from infection. Profound changes in T cell homeostasis raise critical questions about the early events in COVID-19 infection and demonstrate that immune repertoire analysis is a promising method for evaluating emergent host immunity to SARS-CoV-2 viral infection, with great implications for assessing vaccination and other immunological therapies.
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Reviewed by: Yu Nee Lee, Sheba Medical Center, Israel; James M. Heather, Massachusetts General Hospital and Harvard Medical School, United States
These authors have contributed equally to this work
Edited by: Lucia Lopalco, San Raffaele Hospital (IRCCS), Italy
This article was submitted to Viral Immunology, a section of the journal Frontiers in Immunology
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2020.582010