Molecular dynamic simulations of protein/RNA complexes: CRISPR/Csy4 endoribonuclease

• Published in: Biochimica et biophysica acta Vol. 1850; no. 5; pp. 1072 - 1090
• Main Authors: Estarellas, Carolina, Otyepka, Michal, Koča, Jaroslav, Banáš, Pavel, Krepl, Miroslav, Šponer, Jiří
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2015
• Subjects: Binding Sites; Cas6 superfamily; Catalytic Domain; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Proteins - chemistry; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems; Crystallography, X-Ray; Endoribonuclease; Endoribonucleases - chemistry; Endoribonucleases - metabolism; Force field; genome; immune system; Molecular dynamic simulation; molecular dynamics; Molecular Dynamics Simulation; phosphates; Protein Binding; Protein/RNA complex; RNA cleavage; RNA precursors; solvents; Time Factors; X-radiation; Force field; Molecular dynamic simulation; Cas6 superfamily; RNA cleavage; Endoribonuclease; Protein/RNA complex
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2014.10.021

Many prokaryotic genomes comprise Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) offering defense against foreign nucleic acids. These immune systems are conditioned by the production of small CRISPR-derived RNAs matured from long RNA precursors. This often requires a Csy4 endor...