Chitosan-elicited defense responses in Cucumber mosaic virus (CMV)-infected tomato plants

• Published in: Journal of plant physiology Vol. 234-235; pp. 9 - 17
• Main Authors: Rendina, Nunzia, Nuzzaci, Maria, Scopa, Antonio, Cuypers, Ann, Sofo, Adriano
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.03.2019; Elsevier Science Ltd
• Subjects: Accumulation; biochemical pathways; Chitosan; Crop yield; crops; Cucumber mosaic virus; Defense/antioxidant-related protein; Disease control; Flowers & plants; foliar spraying; fruit yield; Gas exchange; Gene expression; genes; Inoculation; Natural environment; Oxidation resistance; Photosynthesis; Photosynthetic performance; plant disease control; Plant diseases; plant diseases and disorders; Plant resistance; Plant virus diseases; Plant viruses; Proteins; Signs and symptoms; Solanum lycopersicum; Solanum lycopersicum var. cerasiforme; Tomatoes; Viral infections; Viruses; DAS-ELISA; PAR; A; Defense/antioxidant-related protein; PR; NPR1; SAR; Photosynthetic performance; ΦPSII; CHT; SA; PVX; ANOVA; UK; Cucumber mosaic virus; Solanum lycopersicum var. cerasiforme; qPCR; TUB; CERK1; ISR; gs; CMV; Disease control; ET; Fv/Fm; JA; PSY; ROS; AR156; PAL; Chitosan; TMV; TP; GAPDH
• DOI: 10.1016/j.jplph.2019.01.003
• ISSN: 0176-1617; 1618-1328

[Display omitted] •Tomato plants chitosan-elicited to verify its Cucumber mosaic virus control efficacy.•Viral titer, plant photosynthetic performance and gene expression were investigated.•Chitosan reduced viral load, improved gas exchange and up-regulated PAL5 expression.•Virus alone did not significantly affect CEVI-1, NPR1, PSY2 and PAL5 expressions.•Chitosan treatment elicited plant defense responses against viral infection. The control of plant diseases by inducing plant resistance responses represents an interesting solution to avoid yield losses and protect the natural environment. Hence, the intertwined relationships between host, pathogen and inducer are increasingly subject of investigations. Here, we report the efficacy of chitosan-elicited defense responses in Solanum lycopersicum var. cerasiforme plants against Cucumber mosaic virus (CMV). Chitosan was applied via foliar spray before the CMV inoculation to verify its effectiveness as a preventive treatment against the viral infection. Virus accumulation, photosynthetic performance, as well as genes encoding for proteins affecting resistance responses and biosynthetic pathways, were investigated. It was observed a significant reduction of CMV accumulation in chitosan-treated plants that were successively infected with CMV, compared to only CMV-infected ones (up to 100%). Similarly, a positive effect of chitosan on gas exchange dynamics was revealed. The analysis of gene expression (CEVI-1, NPR1, PSY2 and PAL5) suggested the occurrence of chitosan-induced, systemic acquired resistance-related responses associated with a readjustment of the plant’s oxidative status. In addition, the absence of deleterious symptoms in chitosan-treated successively CMV-infected plants, confirmed that chitosan can be used as a powerful control agent. Our data indicate that chitosan, when preventively applied, is able to elicit defense responses in tomato to control CMV infection. Such finding may be recommended to protect the tomato fruit yields as well as other crops.