Genotyping by dynamic heating of monolayered beads on a microheated surface

• Published in: Electrophoresis Vol. 25; no. 21-22; pp. 3712 - 3719
• Main Authors: Russom, Aman, Haasl, Sjoerd, Ohlander, Anna, Mayr, Torsten, Brookes, Anthony J., Andersson, Helene, Stemme, Göran
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2004; WILEY‐VCH Verlag
• Subjects: Alleles; Beads; Clinical genetics; Dermatologi och venerologi, klinisk genetik, invärtesmedicin; Dermatology and venerology,clinical genetics, internal medicine; DNA Mutational Analysis - methods; Dynamic allele-specific hybridization; Equipment Design; Genotype; Hot Temperature; Klinisk genetik; Lab-On-A-Chip Devices; MEDICIN; Medicin och hälsovetenskap; MEDICINE; Microchip Analytical Procedures - methods; Microcontact printing; Microheater; Microspheres; Miniaturization; Nucleic Acid Denaturation; Nucleic Acid Hybridization; Oligonucleotides - analysis; Oligonucleotides - genetics; Polymorphism, Single Nucleotide; Single-nucleotide polymorphism

A miniaturized bead‐based dynamic allele‐specific hybridization (DASH) approach for single‐nucleotide polymorphism analysis is presented. Chips with integrated heater and temperature sensors for open‐surface DNA analysis were microfabricated. Microcontact printing using a poly(dimethylsiloxane) (PDM...