Cyclophilin OsCYP20-2 with a novel variant integrates defense and cell elongation for chilling response in rice

• Published in: The New phytologist Vol. 225; no. 6; pp. 2453 - 2467
• Main Authors: Ge, Qiang, Zhang, Yuanyuan, Xu, Yunyuan, Bai, Mingyi, Luo, Wei, Wang, Bo, Niu, Yuda, Zhao, Yuan, Li, Shanshan, Weng, Yuxiang, Wang, Zhiyong, Qian, Qian, Chong, Kang
• Format: Journal Article
• Language: English
• Published: England Wiley 01.03.2020; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: Accumulation; Adaptation; Adaptation, Physiological; Cell differentiation; cell elongation; Chilling; chilling tolerance; Chloroplasts; Cold Temperature; Cooling; Cyclophilins - genetics; Elongation; Localization; Mutants; Nuclei (cytology); Oryza - genetics; OsCYP20‐2; OsFSD2; Plant growth; Plant Proteins - genetics; Protein structure; Reactive oxygen species; Rice; SLR1; Stress; Superoxide dismutase; Survival; cell elongation; chilling tolerance; OsFSD2; OsCYP20-2; SLR1
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.16324

• Coordinating stress defense and plant growth is a survival strategy for adaptation to different environments that contains a series of processes, such as, cell growth, division and differentiation. However, little is known about the coordination mechanism for protein conformation change. • A cyclophilin OsCYP20-2 with a variant interacts with SLENDER RICE1 (SLR1) and OsFSD2 in the nucleus and chloroplasts, respectively, to integrate chilling tolerance and cell elongation in rice (Oryza sativa) (FSD2, Fe-superoxide dismutase 2). • Mass spectrum assay showed that OsNuCYP20-2 localized at the nucleus (nuclear located OsCYP20-2) was a new variant of OsCYP20-2 that truncated 71 amino-acid residues in N-terminal. The loss-of function OsCYP20-2 mutant showed sensitivity to chilling stress with accumulation of extra reactive oxygen species (ROS). In chloroplasts, the full-length OsCYP20-2 promotes OsFSD2 forming homodimers which enhance its activity, eliminating the accumulation of ROS under chilling stress. However, the mutant had shorter epidermal cells in comparison with wild-type Hwayoung (HY). In the nucleus, OsCYP20-2 caused conformation change of SLR1 to promote its degradation for cell elongation. • Our data reveal a cyclophilin with a variant with dual-localization in chloroplasts and the nucleus, which mediate chilling tolerance and cell elongation.