Differences in visible light-induced pigmentation according to wavelengths: a clinical and histological study in comparison with UVB exposure

Summary The visible light spectrum is wide, and it can be hypothesized that all the wavelengths between 400–700 nm do not induce the same photobiological effects on pigmentation. We assessed the potential pro‐pigmenting effects of two single wavelengths located at both extremities of the visible spe...

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Published inPigment cell and melanoma research Vol. 27; no. 5; pp. 822 - 826
Main Authors Duteil, Luc, Cardot-Leccia, Nathalie, Queille-Roussel, Catherine, Maubert, Yves, Harmelin, Yona, Boukari, Fériel, Ambrosetti, Damien, Lacour, Jean-Philippe, Passeron, Thierry
Format Journal Article
LanguageEnglish
Published England Blackwell Publishing Ltd 01.09.2014
Wiley Subscription Services, Inc
Subjects
Colorimetry
Extremities
Healthy Volunteers
Humans
Hyperpigmentation - pathology
Irradiation
Keratinocytes - cytology
Light
Melanocytes - cytology
melanogenesis
Necrosis
p53
Pigmentation
Pigmentation - physiology
Skin - radiation effects
Skin Pigmentation - physiology
Sunscreening Agents
Tumor Suppressor Protein p53 - metabolism
Ultraviolet Rays
UVB
visible light
Wavelengths
UVB
melanogenesis
pigmentation
visible light
p53
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Summary:Summary The visible light spectrum is wide, and it can be hypothesized that all the wavelengths between 400–700 nm do not induce the same photobiological effects on pigmentation. We assessed the potential pro‐pigmenting effects of two single wavelengths located at both extremities of the visible spectrum: the blue/violet line (λ = 415 nm) and the red line (λ = 630 nm). We made colorimetric, clinical, and histological assessments with increasing doses of those lights on healthy volunteers. Then, we compared these irradiations to non‐exposed and UVB‐exposed skin. Colorimetric and clinical assessments showed a clear dose effect with the 415‐nm irradiation, in both skin type III and IV subjects, whereas the 630 nm did not induce hyperpigmentation. When compared to UVB irradiation, the blue–violet light induced a significantly more pronounced hyperpigmentation that lasted up to 3 months. Histological examination showed a significant increase of keratinocyte necrosis and p53 with UVB, as compared to 415‐ and 630‐nm exposures.
Bibliography:ark:/67375/WNG-HTKCLVM8-P
ArticleID:PCMR12273
istex:EB4B230127A727375440541DA10E07C00B5B7D94
Deleo luminotherapy, St Raphael, France
Figure S1. Evolution of the ITA angle as a function of the 415 nm doses [(A) for skin type III, n = 6; (B) for skin type IV, n = 6) and 630 nm doses (C) for skin type III, n = 6; (D) for skin type IV, n = 6).Figure S2. Test zones locations.Figure S3. Immunohistochemical analysis for Oxo-8.Figure S4. Immunohistochemical analysis for p53.Figure S5. Immunohistochemical analysis for MITF.Figure S6. Clinical example of pigmentation induced with increased doses of 415 nm light (10, 30, 60, 90, 110 and 150 J/cm2) in skin type IV subject, 3 months after one single irradiation.Figure S7. Clinical example of pigmentation 3 months after exposure in skin type IV (upper left, 70% blue and 30% red; upper right, non-exposed; lower left, UVB; lower right, 100% blue)Data S1. Methods. Table S1. MED and MPD values as a function of skin types (mean ± SD). Table S2. Synthesis of histology results.
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ISSN:1755-1471
1755-148X
1755-148X
DOI:10.1111/pcmr.12273