Antimicrobial efficacy of drug blended biosynthesized colloidal gold nanoparticles from Justicia glauca against oral pathogens: A nanoantibiotic approach

• Published in: Microbial pathogenesis Vol. 113; pp. 295 - 302
• Main Authors: Emmanuel, Rayappan, Saravanan, Muthupandian, Ovais, Muhammad, Padmavathy, Sethuramasamy, Shinwari, Zabta Khan, Prakash, Periyakaruppan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2017
• Subjects: ambient temperature; antibiotic resistance; antifungal properties; Antimicrobial activity; azithromycin; Bacillus subtilis; bacteria; Candida albicans; clarithromycin; cytotoxicity; drug delivery systems; drug therapy; drugs; energy-dispersive X-ray analysis; Escherichia coli; Fourier transform infrared spectroscopy; fungi; Gold nanoparticles; Green synthesis; ions; Justicia; Justicia glauca; Lactobacillus acidophilus; leaf extracts; Micrococcus luteus; minimum inhibitory concentration; nanogold; Oral pathogens; pathogens; Pseudomonas aeruginosa; Saccharomyces cerevisiae; Staphylococcus aureus; Streptococcus mutans; transmission electron microscopy; ultraviolet-visible spectroscopy; X-ray diffraction; Antimicrobial activity; Green synthesis; Gold nanoparticles; Oral pathogens; Justicia glauca
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2017.10.055

Antimicrobial resistance is a challenging task for researchers to develop new strategies. Green synthesis of AuNPs is an eco-friendly approach, which can be utilized in the microbistatic and microbicidal activities. The current study is focused on Justicia glauca (aqueous leaf extract) mediated AuNPs synthesis at room temperature by treating chloroaurate ions, that shows an antagonistic effect with Azithromycin (AZM) and Clarithromycin (CLR) antibiotics against oral pathogenic bacteria and fungi (Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus, Escherichia coli, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Candida albicans). Characterization of green synthesized AuNPs was done by using Ultraviolet-visible (UV-vis) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD) analysis and Energy Dispersive X-ray analysis (EDAX). Biosynthesized AuNPs were stable, hexagonal and spherical shaped with a size ∼32.5 ± 0.25 nm. The AuNPs and drug conjugated AuNPs showed potential antibacterial and antifungal activity against the oral pathogens. Minimum Inhibitory Concentration (MIC) values of biogenic AuNPs were observed in the range of 6.25–25 μg/mL against selected oral pathogens. Overall, we conclude that biogenic drug delivery system for AZM and CLR can be exploited as potential antimicrobial therapy in future, subject to detailed in-vitro and in-vivo cytotoxicity. Overall scheme of the study for the phytosynthesis of AuNPs, its drug blending and potential synergistic anti-microbial activity. [Display omitted] •Green synthesis of AuNPs from Justicia glauca plant extract.•AuNPs were blended with Azithromycin and Clarithromycin.•AuNPs shows an antagonistic effect with antibiotics against oral pathogens.