Immunization with a low-dose replicon DNA vaccine encoding Phl p 5 effectively prevents allergic sensitization

• Published in: Journal of Allergy and Clinical Immunology Vol. 118; no. 3; pp. 734 - 741
• Main Authors: Gabler, Maximilian, Scheiblhofer, Sandra, Kern, Kerstin, Leitner, Wolfgang W., Stoecklinger, Angelika, Hauser-Kronberger, Cornelia, Alinger, Beate, Lechner, Berta, Prinz, Monika, Vrtala, Susanne, Valenta, Rudolf, Thalhamer, Josef, Weiss, Richard
• Format: Journal Article
• Language: English
• Published: United States Mosby, Inc 01.09.2006; Elsevier Limited
• Subjects: Airway management; alphavirus; Animals; Apoptosis; bronchoalveolar lavage; Cell Line, Tumor; Cells, Cultured; Deoxyribonucleic acid; DNA; DNA vaccine; Dose-Response Relationship, Immunologic; eosinophils; Female; genetic immunization; IgE; Injections, Intradermal; Leukemia; lung pathology; Mice; Mice, Inbred BALB C; Phl p 5; Plant Proteins - administration & dosage; Plant Proteins - genetics; Plant Proteins - immunology; Plasmids; replicase; Replicon - immunology; Respiratory Hypersensitivity - immunology; Respiratory Hypersensitivity - prevention & control; RNA-Dependent RNA Polymerase - genetics; RNA-Dependent RNA Polymerase - immunology; Rodents; Sindbis virus; Sindbis Virus - enzymology; Sindbis Virus - genetics; Sindbis Virus - immunology; Studies; T cell receptors; Th1 Cells - immunology; Type I allergy; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Viral infections; Viral Vaccines - administration & dosage; Viral Vaccines - genetics; Viral Vaccines - immunology; Germany; DNA vaccine; replicase; PAS; Type I allergy; pCMV-P5; IgE; H&E; eosinophils; alphavirus; BAL; pCMV; genetic immunization; Phl p 5; lung pathology; TLR; pSin; pSin-P5; bronchoalveolar lavage

Replicase-based DNA vaccines stimulate T H1-biased immune responses at ultralow doses and induce self-removal of transfected cells through apoptosis. Both aspects are important requirements for efficient and safe DNA-based immunotherapy of type I allergies. A Sindbis virus replicon-based DNA vaccine...