Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary

• Published in: Talanta (Oxford) Vol. 221; p. 121626
• Main Authors: Tůma, Petr, Hložek, Tomáš, Sommerová, Blanka, Koval, Dušan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: acetic acid; acetonitrile; acrylamides; Anticonvulsants; Antiepileptic; area; blood serum; Capillary electrophoresis; chemical bonding; chemical species; Chlorides; Coating; composite polymers; Contactless conductivity detection; electric potential difference; electroosmosis; electrophoresis; Electrophoresis, Capillary; flow; Humans; length; precision; Sample pretreatment; separation; Serum; silica; Stacking; volume; zwitterions; BGE; CE; AcOH; Capillary electrophoresis; Stacking; Antiepileptic; Contactless conductivity detection; LVSS; PGB; VGB; C4D; GBP; Sample pretreatment; Serum; PAMAPTAC; Coating; ID; EOF; PAM; APTAC; FD

Electrophoretic stacking is developed for sensitive determination of three zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, in human serum. CE separation is performed in a 25 μm fused silica capillary covalently coated with the copolymer of acrylamide with 5% content of per...