Fucci2a: A bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice

• Published in: Cell cycle (Georgetown, Tex.) Vol. 13; no. 17; pp. 2681 - 2696
• Main Authors: Mort, Richard Lester, Ford, Matthew Jonathan, Sakaue-Sawano, Asako, Lindstrom, Nils Olof, Casadio, Angela, Douglas, Adam Thomas, Keighren, Margaret Anne, Hohenstein, Peter, Miyawaki, Atsushi, Jackson, Ian James
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02.09.2014
• Subjects: 3T3 Cells; Animals; Cell Cycle; Cell Proliferation; Cell Survival; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Fucci; Fucci2; Fucci2a; G1 Phase; Gene Expression; Genes, Reporter; Humans; Integrases - metabolism; kidney; Kidney - embryology; Luminescent Proteins - metabolism; lung; Lung - embryology; melanoblast; Mice; Mitosis; Morphogenesis; Organ Specificity; Red Fluorescent Protein; Time Factors; Time-Lapse Imaging; T2A, Thosea asigna virus 2A peptide; mAG, Monomeric Azami Green; FACS, Fluorescence-activated cell sorting; Fucci, Fluorescent Ubiquitination-based Cell Cycle Indicator; BrdU, 5-bromo-2′-deoxyuridine; cell cycle; ECACC, European Collection of Cell Cultures; DAPI, 4′, 6-diamidino-2-phenylindole; Fucci2; mKO2, Monomeric Kusabira Orange; EMMA, European Mouse Mutant Archive; Fucci; lung; DMEM, Dulbeccos modified eagle medium; kidney; GMEM, Glasgow minimum essential medium; melanoblast; Fucci2a; mESC, Mouse embryonic stem cell; LIF, leukemia inhibitory factor; RBDB, Riken Bioresource Center DNA Bank; IRES, Internal ribosomal entry site; hESC, Human embryonic stem cell

Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorpor...