Fucci2a: A bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice
Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorpor...
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Published in | Cell cycle (Georgetown, Tex.) Vol. 13; no. 17; pp. 2681 - 2696 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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Taylor & Francis
02.09.2014
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Abstract | Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorporate Fucci are beset by problems with transgene inactivation, varying expression level, lack of conditional potential and/or the need to maintain separate transgenes-there is no transgenic mouse model that solves all these problems. To address these shortfalls we re-engineered the Fucci system to create 2 bicistronic Fucci variants incorporating both probes fused using the Thosea asigna virus 2A (T2A) self cleaving peptide. We characterize these variants in stable 3T3 cell lines. One of the variants (termed Fucci2a) faithfully recapitulated the nuclear localization and cell cycle stage specific florescence of the original Fucci system. We go on to develop a conditional mouse allele (R26Fucci2aR) carefully designed for high, inducible, ubiquitous expression allowing investigation of cell cycle status in single cell lineages within the developing embryo. We demonstrate the utility of R26Fucci2aR for live imaging by using high resolution confocal microscopy of ex vivo lung, kidney and neural crest development. Using our 3T3 system we describe and validate a method to estimate cell cycle times from relatively short time-lapse sequences that we then apply to our neural crest data. The Fucci2a system and the R26Fucci2aR mouse model are compelling new tools for the investigation of cell cycle dynamics in cell culture and during mouse embryonic development. |
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AbstractList | Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorporate Fucci are beset by problems with transgene inactivation, varying expression level, lack of conditional potential and/or the need to maintain separate transgenes-there is no transgenic mouse model that solves all these problems. To address these shortfalls we re-engineered the Fucci system to create 2 bicistronic Fucci variants incorporating both probes fused using the Thosea asigna virus 2A (T2A) self cleaving peptide. We characterize these variants in stable 3T3 cell lines. One of the variants (termed Fucci2a) faithfully recapitulated the nuclear localization and cell cycle stage specific florescence of the original Fucci system. We go on to develop a conditional mouse allele (R26Fucci2aR) carefully designed for high, inducible, ubiquitous expression allowing investigation of cell cycle status in single cell lineages within the developing embryo. We demonstrate the utility of R26Fucci2aR for live imaging by using high resolution confocal microscopy of ex vivo lung, kidney and neural crest development. Using our 3T3 system we describe and validate a method to estimate cell cycle times from relatively short time-lapse sequences that we then apply to our neural crest data. The Fucci2a system and the R26Fucci2aR mouse model are compelling new tools for the investigation of cell cycle dynamics in cell culture and during mouse embryonic development. Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorporate Fucci are beset by problems with transgene inactivation, varying expression level, lack of conditional potential and/or the need to maintain separate transgenes—there is no transgenic mouse model that solves all these problems. To address these shortfalls we re-engineered the Fucci system to create 2 bicistronic Fucci variants incorporating both probes fused using the Thosea asigna virus 2A (T2A) self cleaving peptide. We characterize these variants in stable 3T3 cell lines. One of the variants (termed Fucci2a) faithfully recapitulated the nuclear localization and cell cycle stage specific florescence of the original Fucci system. We go on to develop a conditional mouse allele ( R26Fucci2aR ) carefully designed for high, inducible, ubiquitous expression allowing investigation of cell cycle status in single cell lineages within the developing embryo. We demonstrate the utility of R26Fucci2aR for live imaging by using high resolution confocal microscopy of ex vivo lung, kidney and neural crest development. Using our 3T3 system we describe and validate a method to estimate cell cycle times from relatively short time-lapse sequences that we then apply to our neural crest data. The Fucci2a system and the R26Fucci2aR mouse model are compelling new tools for the investigation of cell cycle dynamics in cell culture and during mouse embryonic development. |
Author | Keighren, Margaret Anne Ford, Matthew Jonathan Casadio, Angela Douglas, Adam Thomas Mort, Richard Lester Lindstrom, Nils Olof Sakaue-Sawano, Asako Jackson, Ian James Hohenstein, Peter Miyawaki, Atsushi |
Author_xml | – sequence: 1 givenname: Richard Lester surname: Mort fullname: Mort, Richard Lester organization: MRC Human Genetics Unit; MRC IGMM; University of Edinburgh; Western General Hospital Edinburgh – sequence: 2 givenname: Matthew Jonathan surname: Ford fullname: Ford, Matthew Jonathan organization: MRC Human Genetics Unit; MRC IGMM; University of Edinburgh; Western General Hospital Edinburgh – sequence: 3 givenname: Asako surname: Sakaue-Sawano fullname: Sakaue-Sawano, Asako organization: Laboratory for Cell Function and Dynamics; Advanced Technology Development Group; Brain Science Institute; RIKEN – sequence: 4 givenname: Nils Olof surname: Lindstrom fullname: Lindstrom, Nils Olof organization: The Roslin Institute; The University of Edinburgh; Easter Bush, Midlothian – sequence: 5 givenname: Angela surname: Casadio fullname: Casadio, Angela organization: MRC Human Genetics Unit; MRC IGMM; University of Edinburgh; Western General Hospital Edinburgh – sequence: 6 givenname: Adam Thomas surname: Douglas fullname: Douglas, Adam Thomas organization: MRC Human Genetics Unit; MRC IGMM; University of Edinburgh; Western General Hospital Edinburgh – sequence: 7 givenname: Margaret Anne surname: Keighren fullname: Keighren, Margaret Anne organization: MRC Human Genetics Unit; MRC IGMM; University of Edinburgh; Western General Hospital Edinburgh – sequence: 8 givenname: Peter surname: Hohenstein fullname: Hohenstein, Peter organization: The Roslin Institute; The University of Edinburgh; Easter Bush, Midlothian – sequence: 9 givenname: Atsushi surname: Miyawaki fullname: Miyawaki, Atsushi organization: Laboratory for Cell Function and Dynamics; Advanced Technology Development Group; Brain Science Institute; RIKEN – sequence: 10 givenname: Ian James surname: Jackson fullname: Jackson, Ian James email: Ian.Jackson@igmm.ed.ac.uk organization: The Roslin Institute; The University of Edinburgh; Easter Bush, Midlothian |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25486356$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1016/j.stem.2008.12.003 10.1016/S0925-4773(97)00188-3 10.1038/nmeth.2019 10.1101/pdb.ip067876 10.1089/scd.2009.0065 10.1016/j.cell.2007.12.033 10.1002/dvg.20335 10.1186/1471-213X-1-4 10.1002/jcb.10712 10.1038/jid.2012.186 10.1002/(SICI)1097-0177(199810)213:2<228::AID-AJA8>3.0.CO;2-I 10.1111/j.1755-148X.2010.00669.x 10.1006/dbio.2001.0167 10.1016/j.scr.2012.10.004 10.1099/0022-1317-78-1-13 10.1038/5007 10.1016/j.stemcr.2013.10.009 10.1101/gad.5.9.1513 10.1016/j.devcel.2011.07.008 10.1007/BF01190834 10.1016/j.ydbio.2005.04.005 10.1038/nmeth.2089 10.1038/nature02381 10.1111/j.0021-8782.2004.00285.x 10.2144/000112243 10.1016/j.ydbio.2006.08.035 10.1016/S0168-9525(97)01097-4 10.1016/0092-8674(91)90071-6 10.1371/journal.pbio.1000420 10.1016/0378-1119(89)90209-6 10.4161/cc.4.9.2048 10.1016/j.cell.2013.08.031 10.1038/sj.gt.3303065 10.1073/pnas.88.11.4671 10.1016/0955-0674(95)80065-4 10.1152/physiolgenomics.2000.2.2.67 10.1099/0022-1317-82-5-1027 10.1074/jbc.M312644200 10.1016/j.cell.2005.02.012 10.1016/j.cub.2004.09.020 10.1242/dev.084111 10.1186/1471-2121-12-2 10.1371/journal.pone.0023376 10.1186/1755-8417-1-3 10.1371/journal.pone.0000001 10.1242/dev.067447 10.1242/dev.098855 10.1101/gad.891101 |
ContentType | Journal Article |
Copyright | 2014 The Author(s). Published with license by Taylor & Francis Group, LLC © Richard Lester Mort, Matthew Jonathan Ford, Asako Sakaue-Sawano, Nils Olof Lindstrom, Angela Casadio, Adam Thomas Douglas, Margaret Anne Keighren, Peter Hohenstein, Atsushi Miyawaki, and Ian James Jackson 2014 2014 The Author(s). Published with license by Taylor & Francis Group, LLC © Richard Lester Mort, Matthew Jonathan Ford, Asako Sakaue-Sawano, Nils Olof Lindstrom, Angela Casadio, Adam Thomas Douglas, Margaret Anne Keighren, Peter Hohenstein, Atsushi Miyawaki, and Ian James Jackson 2014 The Author(s) |
Copyright_xml | – notice: 2014 The Author(s). Published with license by Taylor & Francis Group, LLC © Richard Lester Mort, Matthew Jonathan Ford, Asako Sakaue-Sawano, Nils Olof Lindstrom, Angela Casadio, Adam Thomas Douglas, Margaret Anne Keighren, Peter Hohenstein, Atsushi Miyawaki, and Ian James Jackson 2014 – notice: 2014 The Author(s). Published with license by Taylor & Francis Group, LLC © Richard Lester Mort, Matthew Jonathan Ford, Asako Sakaue-Sawano, Nils Olof Lindstrom, Angela Casadio, Adam Thomas Douglas, Margaret Anne Keighren, Peter Hohenstein, Atsushi Miyawaki, and Ian James Jackson 2014 The Author(s) |
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Keywords | T2A, Thosea asigna virus 2A peptide mAG, Monomeric Azami Green FACS, Fluorescence-activated cell sorting Fucci, Fluorescent Ubiquitination-based Cell Cycle Indicator BrdU, 5-bromo-2′-deoxyuridine cell cycle ECACC, European Collection of Cell Cultures DAPI, 4′, 6-diamidino-2-phenylindole Fucci2 mKO2, Monomeric Kusabira Orange EMMA, European Mouse Mutant Archive Fucci lung DMEM, Dulbeccos modified eagle medium kidney GMEM, Glasgow minimum essential medium melanoblast Fucci2a mESC, Mouse embryonic stem cell LIF, leukemia inhibitory factor RBDB, Riken Bioresource Center DNA Bank IRES, Internal ribosomal entry site hESC, Human embryonic stem cell |
Language | English |
License | open-access: http://creativecommons.org/licenses/by-nc/3.0/: This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
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References | 25807308 - Cell Cycle. 2015;14(7):948-9 cit0033 cit0034 cit0031 cit0030 Evans V (cit0016) 2000; 2 cit0039 Ang XL (cit0010) 2004; 2004 cit0037 cit0038 cit0035 cit0036 cit0023 cit0020 cit0021 cit0028 cit0029 cit0026 cit0027 cit0024 cit0025 cit0011 cit0012 cit0051 cit0052 cit0050 cit0019 cit0017 cit0018 cit0015 cit0013 cit0014 cit0044 cit0001 cit0045 cit0042 cit0043 cit0040 cit0041 Fiering S (cit0022) 1997 cit0008 cit0009 cit0006 cit0007 cit0004 cit0048 cit0005 cit0049 cit0002 cit0046 cit0003 cit0047 |
References_xml | – ident: cit0041 doi: 10.1016/j.stem.2008.12.003 – start-page: 94 year: 1997 ident: cit0022 publication-title: Proc Natl Acad Sci U S A contributor: fullname: Fiering S – ident: cit0048 doi: 10.1016/S0925-4773(97)00188-3 – ident: cit0051 doi: 10.1038/nmeth.2019 – ident: cit0020 doi: 10.1101/pdb.ip067876 – ident: cit0038 doi: 10.1089/scd.2009.0065 – ident: cit0011 doi: 10.1016/j.cell.2007.12.033 – ident: cit0025 doi: 10.1002/dvg.20335 – ident: cit0024 doi: 10.1186/1471-213X-1-4 – ident: cit0036 doi: 10.1002/jcb.10712 – ident: cit0044 doi: 10.1038/jid.2012.186 – ident: cit0002 doi: 10.1002/(SICI)1097-0177(199810)213:2<228::AID-AJA8>3.0.CO;2-I – ident: cit0033 doi: 10.1111/j.1755-148X.2010.00669.x – ident: cit0043 doi: 10.1002/(SICI)1097-0177(199810)213:2<228::AID-AJA8>3.0.CO;2-I – ident: cit0046 doi: 10.1006/dbio.2001.0167 – ident: cit0037 doi: 10.1016/j.scr.2012.10.004 – ident: cit0019 doi: 10.1099/0022-1317-78-1-13 – ident: cit0015 doi: 10.1038/5007 – ident: cit0039 doi: 10.1016/j.stemcr.2013.10.009 – ident: cit0023 doi: 10.1101/gad.5.9.1513 – ident: cit0045 doi: 10.1016/j.devcel.2011.07.008 – ident: cit0030 doi: 10.1007/BF01190834 – ident: cit0029 doi: 10.1016/j.ydbio.2005.04.005 – ident: cit0052 doi: 10.1038/nmeth.2089 – ident: cit0007 doi: 10.1038/nature02381 – ident: cit0003 doi: 10.1111/j.0021-8782.2004.00285.x – ident: cit0018 doi: 10.2144/000112243 – ident: cit0031 doi: 10.1016/j.ydbio.2006.08.035 – ident: cit0013 doi: 10.1016/S0168-9525(97)01097-4 – ident: cit0049 doi: 10.1016/0092-8674(91)90071-6 – ident: cit0004 doi: 10.1371/journal.pbio.1000420 – ident: cit0021 doi: 10.1016/0378-1119(89)90209-6 – volume: 2004 start-page: pe31 year: 2004 ident: cit0010 publication-title: Sci STKE contributor: fullname: Ang XL – ident: cit0008 doi: 10.4161/cc.4.9.2048 – ident: cit0040 doi: 10.1016/j.cell.2013.08.031 – ident: cit0042 doi: 10.1038/sj.gt.3303065 – ident: cit0047 doi: 10.1073/pnas.88.11.4671 – ident: cit0001 doi: 10.1016/0955-0674(95)80065-4 – volume: 2 start-page: 67 year: 2000 ident: cit0016 publication-title: Physiol Genomics doi: 10.1152/physiolgenomics.2000.2.2.67 contributor: fullname: Evans V – ident: cit0035 doi: 10.1099/0022-1317-82-5-1027 – ident: cit0009 doi: 10.1074/jbc.M312644200 – ident: cit0026 doi: 10.1016/j.cell.2005.02.012 – ident: cit0006 doi: 10.1016/j.cub.2004.09.020 – ident: cit0014 doi: 10.1242/dev.084111 – ident: cit0012 doi: 10.1186/1471-2121-12-2 – ident: cit0027 doi: 10.1371/journal.pone.0023376 – ident: cit0050 doi: 10.1186/1755-8417-1-3 – ident: cit0028 doi: 10.1371/journal.pone.0000001 – ident: cit0034 doi: 10.1242/dev.067447 – ident: cit0005 doi: 10.1242/dev.098855 – ident: cit0017 doi: 10.1101/gad.891101 |
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Snippet | Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically... |
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SubjectTerms | 3T3 Cells Animals Cell Cycle Cell Proliferation Cell Survival Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Fucci Fucci2 Fucci2a G1 Phase Gene Expression Genes, Reporter Humans Integrases - metabolism kidney Kidney - embryology Luminescent Proteins - metabolism lung Lung - embryology melanoblast Mice Mitosis Morphogenesis Organ Specificity Red Fluorescent Protein Time Factors Time-Lapse Imaging |
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Title | Fucci2a: A bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice |
URI | https://www.tandfonline.com/doi/abs/10.4161/15384101.2015.945381 https://www.ncbi.nlm.nih.gov/pubmed/25486356 https://search.proquest.com/docview/1635002176 https://pubmed.ncbi.nlm.nih.gov/PMC4613862 |
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