In vitro Eye Irritancy Test of Lauryl Derivatives and Polyoxyethylene Alkyl Derivatives with the Reconstructed Rabbit Corneal Epithelium Model

• Published in: Journal of Oleo Science Vol. 58; no. 8; pp. 437 - 442
• Main Authors: Matsuda, Sanae, Hisama, Masayoshi, Shibayama, Hiroharu, Itou, Norihiko, Iwaki, Masahiro
• Format: Journal Article
• Language: English
• Published: Japan Japan Oil Chemists' Society 2009; Japan Science and Technology Agency
• Subjects: Animals; Cell Survival - drug effects; Cells, Cultured; cosmetic integrants; Dose-Response Relationship, Drug; Epithelium, Corneal - cytology; Epithelium, Corneal - drug effects; Epithelium, Corneal - pathology; eye irritation; Irritants - pharmacology; Irritants - toxicity; Laurates - pharmacology; Laurates - toxicity; Models, Biological; MTT; Polyethylene Glycols - pharmacology; rabbit corneal epithelial cell; rabbit corneal epithelium model; Rabbits; Structure-Activity Relationship

The rabbit corneal epithelium model (RCE model) was developed as a three-dimensional in vitro model to replace animal testing for the assessment of eye tolerance. In the model, a stratified culture of rabbit corneal epithelial cells is grown at the air-liquid interface on a collagen gel acting as a...