Virtual karyotyping of pluripotent stem cells on the basis of their global gene expression profiles

• Published in: Nature protocols Vol. 8; no. 5; pp. 989 - 997
• Main Authors: Ben-David, Uri, Mayshar, Yoav, Benvenisty, Nissim
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.05.2013; Nature Publishing Group
• Subjects: 38/61; 45/100; 631/114; 631/1647/1407/1405; 631/1647/2217/2018; 631/532/2064; Analytical Chemistry; Biological Techniques; Chromosome Aberrations; Chromosome abnormalities; Chromosomes; Computational Biology; Computational Biology/Bioinformatics; Databases, Genetic; Deoxyribonucleic acid; DNA; Gene expression; Genetic aspects; Genetic engineering; Genomes; Genomic Instability; Genomics; Karyotyping; Karyotyping - methods; Laboratories; Life Sciences; Methods; Microarrays; Oligonucleotide Array Sequence Analysis; Organic Chemistry; Physiological aspects; Pluripotent Stem Cells; Protocol; Risk factors; Stem cell research; Stem cells; Transcriptome; United States

The genomic instability of stem cells in culture, caused by their routine in vitro propagation or by their genetic manipulation, is deleterious both for their clinical application and for their use in basic research. Frequent evaluation of the genomic integrity of stem cells is thus required, and it...