Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer

Cancer cells release extracellular vesicles, which are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy. Size-exclusion chromatography (SEC) is an increasingly popular technique, which has been rediscovered for the purposes of extracellular vesicle (EV) isola...

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Published inCancers Vol. 12; no. 11; p. 3156
Main Authors Liu, Daniel S. K., Upton, Flora M., Rees, Eleanor, Limb, Christopher, Jiao, Long R., Krell, Jonathan, Frampton, Adam E.
Format Journal Article
LanguageEnglish
Published MDPI AG 01.11.2020
MDPI
Subjects
biomarkers
Cancer
Diagnosis
exosomes
extracellular vesicles
Gel permeation chromatography
Methods
Review
SEC
size-exclusion chromatography
United Kingdom
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Abstract Cancer cells release extracellular vesicles, which are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy. Size-exclusion chromatography (SEC) is an increasingly popular technique, which has been rediscovered for the purposes of extracellular vesicle (EV) isolation and purification from diverse biofluids. A systematic review was undertaken to identify all papers that described size exclusion as their primary EV isolation method in cancer research. In all, 37 papers were identified and discussed, which showcases the breadth of applications in which EVs can be utilised, from proteomics, to RNA, and through to functionality. A range of different methods are highlighted, with Sepharose-based techniques predominating. EVs isolated using SEC are able to identify cancer cells, highlight active pathways in tumourigenesis, clinically distinguish cohorts, and remain functionally active for further experiments.
AbstractList Extracellular vesicles (EVs) are small particles that are released by cancer cells, and they may hold vital information for researchers looking for early markers for diagnosis. Size-exclusion chromatography (SEC) is a classical technique that has become increasingly popular and can be used for rapid isolation and investigation of both their cargo and functionality. This systematic review highlights its main technical aspects, the type of materials involved and by covering the findings of the identified papers hopes to demonstrate the utility of this method in cancer research to date.
Cancer cells release extracellular vesicles, which are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy. Size-exclusion chromatography (SEC) is an increasingly popular technique, which has been rediscovered for the purposes of extracellular vesicle (EV) isolation and purification from diverse biofluids. A systematic review was undertaken to identify all papers that described size exclusion as their primary EV isolation method in cancer research. In all, 37 papers were identified and discussed, which showcases the breadth of applications in which EVs can be utilised, from proteomics, to RNA, and through to functionality. A range of different methods are highlighted, with Sepharose-based techniques predominating. EVs isolated using SEC are able to identify cancer cells, highlight active pathways in tumourigenesis, clinically distinguish cohorts, and remain functionally active for further experiments.
Audience Academic
Author Limb, Christopher
Frampton, Adam E.
Liu, Daniel S. K.
Jiao, Long R.
Rees, Eleanor
Upton, Flora M.
Krell, Jonathan
AuthorAffiliation 2 Hepato-Pancreato-Biliary (HPB) Surgical Unit, Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0HS, UK; l.jiao@imperial.ac.uk
1 Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0HS, UK; daniel.liu08@imperial.ac.uk (D.S.K.L.); f.upton19@imperial.ac.uk (F.M.U.); e.rees@imperial.ac.uk (E.R.); j.krell@imperial.ac.uk (J.K.)
3 Hepato-Pancreato-Biliary (HPB) Surgical Unit, Royal Surrey County Hospital NHS Foundation Trust, Guildford, Surrey GU2 7XX, UK; c.limb@surrey.ac.uk
4 Department of Clinical and Experimental Medicine, Faculty of Health and Medical Sciences, The Leggett Building, University of Surrey, Guildford, Surrey GU2 7WG, UK
AuthorAffiliation_xml – name: 1 Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0HS, UK; daniel.liu08@imperial.ac.uk (D.S.K.L.); f.upton19@imperial.ac.uk (F.M.U.); e.rees@imperial.ac.uk (E.R.); j.krell@imperial.ac.uk (J.K.)
– name: 2 Hepato-Pancreato-Biliary (HPB) Surgical Unit, Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0HS, UK; l.jiao@imperial.ac.uk
– name: 4 Department of Clinical and Experimental Medicine, Faculty of Health and Medical Sciences, The Leggett Building, University of Surrey, Guildford, Surrey GU2 7WG, UK
– name: 3 Hepato-Pancreato-Biliary (HPB) Surgical Unit, Royal Surrey County Hospital NHS Foundation Trust, Guildford, Surrey GU2 7XX, UK; c.limb@surrey.ac.uk
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Snippet Cancer cells release extracellular vesicles, which are a rich target for biomarker discovery and provide a promising mechanism for liquid biopsy....
Extracellular vesicles (EVs) are small particles that are released by cancer cells, and they may hold vital information for researchers looking for early...
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StartPage 3156
SubjectTerms biomarkers
Cancer
Diagnosis
exosomes
extracellular vesicles
Gel permeation chromatography
Methods
Review
SEC
size-exclusion chromatography
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Title Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer
URI https://search.proquest.com/docview/2456415371
https://pubmed.ncbi.nlm.nih.gov/PMC7693800
https://doaj.org/article/f145afac9c3f4e1b9ef6cdae3435a633
Volume 12
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