Human occult loiasis: improvement in diagnostic sensitivity by the use of a nested polymerase chain reaction

• Published in: The American journal of tropical medicine and hygiene Vol. 59; no. 1; pp. 144 - 149
• Main Authors: Toure, FS, Kassambara, L, Williams, T, Millet, P, Bain, O, Georges, AJ, Egwang, TG
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lawrence, KS ASTMH 01.07.1998; Allen Press
• Subjects: Animals; Biological and medical sciences; Diseases caused by nematodes; DNA Primers - chemistry; DNA, Helminth - blood; Electrophoresis, Agar Gel; Filariases; Helminth Proteins - genetics; Helminthic diseases; Humans; Infectious diseases; Loa - genetics; Loa - isolation & purification; Loaiasis; Loiasis - diagnosis; Medical sciences; Parasitic diseases; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Tropical medicine; Uganda; Africa; Infection; Human; Polymerase chain reaction; Nematode disease; Microfilaria; Filariosis; Parasitosis; Diagnosis; Helminthiasis; Loaiasis

The development of control strategies for loiasis is of crucial importance in endemic areas and depends heavily on the accurate identification of occult-infected individuals. A polymerase chain reaction (PCR) and nested polymerase chain reaction (nested PCR) were developed and based on sequences of...