The Glycosyl Phosphatidylinositol Anchor Is Critical for Ly-6A/E-Mediated T Cell Activation

• Published in: The Journal of cell biology Vol. 112; no. 3; pp. 377 - 384
• Main Authors: Su, Bing, Waneck, Gerald L., Flavell, Richard A., Alfred L. M. Bothwell
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.02.1991; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Animals; Antibodies; Antigenic determinants, haptens, artificial antigens; Antigens; Base Sequence; Biological and medical sciences; Cell Line; Cell lines; Cell Membrane - immunology; Cells; COS cells; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycolipids - immunology; Glycosylphosphatidylinositols; GPI linked proteins; Isoantigens - genetics; Isoantigens - immunology; Lymphocyte Activation; lymphocytes T; Lymphokines - metabolism; Membrane proteins; Molecular immunology; Molecular Sequence Data; Molecules; Mutagenesis, Site-Directed; phosphatidylinositol; Phosphatidylinositols - immunology; Plasmids; Surface antigens; T lymphocytes; T-Lymphocytes - immunology; T-Lymphocytes, Helper-Inducer - immunology; Transfection; Transmembrane protein; Monkey; Activation; Gene expression; Kidney; Antigen; Vertebrata; Mammalia; Alloantigen; Cell line; Transfection; T-Lymphocyte; Primates

Ly-6E, a glycosyl phosphatidylinositol (GPI)-anchored murine alloantigen that can activate T cells upon antibody cross-linking, has been converted into an integral membrane protein by gene fusion. This fusion product, designated Ly-6EDb, was characterized in transiently transfected COS cells and dem...