Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products

• Published in: BMB reports Vol. 48; no. 2; pp. 103 - 108
• Main Authors: Song, M.J., Yonsei University College of Medicine, Seoul, Republic of Korea, Lee, J.J., Yonsei University College of Medicine, Seoul, Republic of Korea, Nam, Y.H., Yonsei University College of Medicine, Seoul, Republic of Korea, Kim, T.G., Yonsei University College of Medicine, Seoul, Republic of Korea, Chung, Y.W., Yonsei University College of Medicine, Seoul, Republic of Korea, Kim, M., Yonsei University College of Medicine, Seoul, Republic of Korea, Choi, Y.E., Yonsei University College of Medicine, Seoul, Republic of Korea, Shin, M.H., Yonsei University College of Medicine, Seoul, Republic of Korea, Kim, H.P., Yonsei University College of Medicine, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.02.2015; 생화학분자생물학회
• Subjects: Animals; Antigens, Protozoan - immunology; Antigens, Protozoan - metabolism; Antigens, Protozoan - pharmacology; Bone Marrow Cells - cytology; Cell Differentiation; CHROMATIN; Chromatin Immunoprecipitation; CHROMATINE; CITOQUINAS; CROMATINA; CYTOKINE; CYTOKINES; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Dendritic cells,Trichomonas vaginalis; Down-Regulation - drug effects; Endopeptidase K - metabolism; Female; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Interleukin-10; Interleukin-12; Lipopolysaccharides - toxicity; Mice; Mice, Inbred C57BL; TOLERANCE; TOLERANCIA; Trichomonas vaginalis - metabolism; Up-Regulation - drug effects; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2015.48.2.116

Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naive T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.