An evaluation of the protective effect of esomeprazole in an experimental model of renal ischemia–reperfusion

• Published in: International urology and nephrology Vol. 50; no. 2; pp. 217 - 223
• Main Authors: Ozkan, Tayyar Alp, Karakoyunlu, Nihat, Polat, Reyhan, Sarıbaş, Gülistan Sanem, Şener, Nevzat Can, Özdemir, Seyda, Peker, Kevser, Ünal, Dilek, Tuygun, Can
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2018; Springer Nature B.V
• Subjects: Antioxidants; Apoptosis; Ischemia; Kidneys; Malondialdehyde; Medicine; Medicine & Public Health; Nephrectomy; Nephrology; Omeprazole; Parenchyma; Peroxidase; Renal artery; Reperfusion; Rodents; Statistical analysis; Superoxide dismutase; Tumors; Urology; Urology - Original Paper; Proton pump inhibitors; Ischemia–reperfusion injury; Rat; Esomeprazole
• DOI: 10.1007/s11255-017-1775-8
• ISSN: 0301-1623

Purpose The ischemia and subsequent reperfusion (IR) which occurs in partial nephrectomy used in the treatment of renal tumors causes loss of parenchyma in the damaged kidney. The aim of this study is to evaluate, both biochemically and histologically, the efficacy of esomeprazole in an ischemia–reperfusion model in rat kidneys. Methods The rats were randomized into three groups of seven animals each, referred to as the sham, control, and PPI groups. In the sham group, only a laparotomy was performed. In the control group, following laparotomy the left renal artery was dissected and tied for 30-min ischemia. In the PPI group, a vascular route to the tail vein was opened, and 10 mg/kg esomeprazole was administered. After 1 h, the same procedures described for the control group were performed. All the animals were killed 24 h after the procedure. Biochemical analyses were applied for evaluation of oxidant and antioxidant agents in the blood and left kidney of each subject (oxidative markers: malondialdehyde, myeloperoxidase; antioxidant marker: superoxide dismutase). In the histological examination of the kidney tissues stained with hematoxylin–eosin, the TUNEL method was applied in the evaluation of apoptosis. Results No statistically significant biochemical difference was determined in the blood and tissue samples. In the histological and apoptosis evaluations, a statistically significant difference was determined between the sham, control, and PPI groups. The median (IQR) values of the TUNEL-positive cells were counted as 1.50 (4) in the sham group, 11.50 (12) in the control group, and 6.00 (9) in the PPI group ( p  < 0.001). Conclusions A protective effect of esomeprazole was confirmed in renal ischemia–reperfusion damage created in an experimental rat model.